Tissue engineered bone grafts based on biomimetic nanocomposite PLGA/amorphous calcium phosphate scaffold and human adipose-derived stem cells

Autor: Sonja Hemmi, Manfred Welti, Nicole Lindenblatt, Clément M. L. Werner, Guido A. Wanner, Oliver D. Schneider, Nora Hild, Luc Härter, Johanna Buschmann, Shuping Gao, Maurizio Calcagni, Wendelin J. Stark
Přispěvatelé: University of Zurich, Buschmann, Johanna
Rok vydání: 2012
Předmět:
Calcium Phosphates
Male
Scaffold
Adipose tissue
Biocompatible Materials
610 Medicine & health
macromolecular substances
Cell morphology
Nanocomposites
chemistry.chemical_compound
2732 Orthopedics and Sports Medicine
Polylactic Acid-Polyglycolic Acid Copolymer
Tissue engineering
Osteogenesis
In vivo
Humans
Medicine
Lactic Acid
Amorphous calcium phosphate
10266 Clinic for Reconstructive Surgery
Cell Proliferation
General Environmental Science
Bone Transplantation
Osteoblasts
Tissue Engineering
Tissue Scaffolds
business.industry
technology
industry
and agriculture
10021 Department of Trauma Surgery
PLGA
10022 Division of Surgical Research
Adipose Tissue
chemistry
Biophysics
Nanoparticles
General Earth and Planetary Sciences
Female
Stem cell
2711 Emergency Medicine
business
Polyglycolic Acid
Stem Cell Transplantation
Zdroj: Injury. 43:1689-1697
ISSN: 0020-1383
DOI: 10.1016/j.injury.2012.06.004
Popis: For tissue engineering of critical size bone grafts, nanocomposites are getting more and more attractive due to their controllable physical and biological properties. We report in vitro and in vivo behaviour of an electrospun nanocomposite based on poly-lactic-co-glycolic acid and amorphous calcium phosphate nanoparticles (PLGA/a-CaP) seeded with human adipose-derived stem cells (ASC) compared to PLGA. Major findings were that cell attachment, three-dimensional ingrowth and proliferation were very good on both materials. Cell morphology changed from a spindle-shaped fibroblast-like form to a more roundish type when ASC were seeded on PLGA, while they retained their morphology on PLGA/a-CaP. Moreover, we found ASC differentiation to a phenotype committed towards osteogenesis when a-CaP nanoparticles were suspended in normal culture medium without any osteogenic supplements, which renders a-CaP nanoparticles an interesting osteoinductive component for the synthesis of other nanocomposites than PLGA/a-CaP. Finally, electrospun PLGA/a-CaP scaffold architecture is suitable for a rapid and homogenous vascularisation confirmed by a complete penetration by avian vessels from the chick chorioallantoic membrane (CAM) within one week.
Databáze: OpenAIRE
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