Brain Aggregates: An EffectiveIn VitroCell Culture System Modeling Neurodegenerative Diseases
| Autor: | Franck Kalume, Abby Oehler, Stephen J. DeArmond, George A. Carlson, Misol Ahn, Rose Pitstick |
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| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine Drug media_common.quotation_subject Cell Culture Techniques Action Potentials Mice Transgenic tau Proteins Disease Biology Models Biological Pathology and Forensic Medicine Mice 03 medical and health sciences Cellular and Molecular Neuroscience 0302 clinical medicine Pregnancy In vivo medicine Animals Humans Cells Cultured media_common Neurons Errata Drug discovery Neurodegeneration Age Factors Brain Neurodegenerative Diseases Original Articles General Medicine Embryo Mammalian medicine.disease Embryonic stem cell In vitro 030104 developmental biology Neurology Cell culture Female Neurology (clinical) Nerve Net Neuroglia Neuroscience 030217 neurology & neurosurgery Sodium Channel Blockers |
| Zdroj: | Journal of Neuropathology & Experimental Neurology. 75:256-262 |
| ISSN: | 1554-6578 0022-3069 |
| DOI: | 10.1093/jnen/nlv025 |
| Popis: | Drug discovery for neurodegenerative diseases is particularly challenging because of the discrepancies in drug effects between in vitro and in vivo studies. These discrepancies occur in part because current cell culture systems used for drug screening have many limitations. First, few cell culture systems accurately model human aging or neurodegenerative diseases. Second, drug efficacy may differ between dividing and stationary cells, the latter resembling nondividing neurons in the CNS. Brain aggregates (BrnAggs) derived from embryonic day 15 gestation mouse embryos may represent neuropathogenic processes in prion disease and reflect in vivo drug efficacy. Here, we report a new method for the production of BrnAggs suitable for drug screening and suggest that BrnAggs can model additional neurological diseases such as tauopathies. We also report a functional assay with BrnAggs by measuring electrophysiological activities. Our data suggest that BrnAggs could serve as an effective in vitro cell culture system for drug discovery for neurodegenerative diseases. |
| Databáze: | OpenAIRE |
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