Virulence genes of Helicobacter pylori in the Dominican Republic
| Autor: | Takahiro Mitsui, Lourdes Tronilo, Modesto Cruz, Mildre Disla, José A. Jiménez Abreu, Yoshio Yamaoka, Eduardo Rodríguez, Hiroyuki Nagashima, Miyuki Matsuda, Hideo Terao, Tomohisa Uchida, Shun Iwatani, Seiji Shiota |
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| Rok vydání: | 2014 |
| Předmět: |
Adult
Male Microbiology (medical) Peptic Ulcer medicine.medical_specialty Adolescent Epidemiology Virulence Factors Molecular Sequence Data Rapid urease test Polymerase Chain Reaction Microbiology Gastroenterology Helicobacter Infections Young Adult Bacterial Proteins Stomach Neoplasms Internal medicine Genotype Prevalence medicine Humans CagA Risk factor Aged Aged 80 and over Antigens Bacterial Helicobacter pylori biology business.industry Incidence (epidemiology) Dominican Republic Cancer Sequence Analysis DNA General Medicine Middle Aged bacterial infections and mycoses medicine.disease biology.organism_classification digestive system diseases Female Gastritis medicine.symptom business |
| Zdroj: | Journal of Medical Microbiology. 63:1189-1196 |
| ISSN: | 1473-5644 0022-2615 |
| Popis: | Although the incidence of gastric cancer in the Dominican Republic is not high, the disease remains a significant health problem. We first conducted a detailed analysis ofHelicobacter pyloristatus in the Dominican Republic. In total, 158 patients (103 females and 55 males; mean age 47.1±16.2 years) were recruited. The status ofH. pyloriinfection was determined based on four tests: rapid urease test, culture test, histological test and immunohistochemistry. The status ofcagAandvacAgenotypes inH. pyloriwas examined using PCR and gene sequencing. The overall prevalence ofH. pyloriinfection was 58.9 %. No relationship was found between theH. pyloriinfection rate and the age range of 17–91 years. Even in the youngest group (patients aged H. pyloriinfection rate was 62.5 %. Peptic ulcer was found in 23 patients and gastric cancer was found in one patient. TheH. pyloriinfection rate in patients with peptic ulcer was significantly higher than that in patients with gastritis (82.6 versus 54.5 %,PcagA-positive/vacAs1m1 genotype was the most prevalent (43/64, 67.2 %). Compared withH. pylori-negative patients,H. pylori-positive patients showed more severe gastritis. Furthermore, the presence ofcagAwas related to the presence of more severe gastritis. All CagA-positive strains had Western-type CagA. In conclusion, we found thatH. pyloriinfection is a risk factor for peptic ulcer in the Dominican Republic. Patients withcagA-positiveH. pyloricould be at higher risk for severe inflammation and atrophy. |
| Databáze: | OpenAIRE |
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