Neuroprotective effect of immunomodulatory peptides in rats with traumatic spinal cord injury
| Autor: | Adrian Flores-Romero, Antonio Ibarra, Dulce Parra-Villamar, Soria Zavala Karla, Liliana Blancas-Espinoza, Juan Herrera-García, Raúl Silva-García, Alberto Toscano-Zapien, Jonathan Vilchis Villa, Elisa García-Vences, Rodríguez Barrera-Roxana |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty medicine.medical_treatment H&E stain Neuroprotection Luxol fast blue stain lcsh:RC346-429 Masson's trichrome stain 03 medical and health sciences 0302 clinical medicine Developmental Neuroscience Parenchyma Medicine monocyte locomotion inhibitor factor Spinal cord injury lcsh:Neurology. Diseases of the nervous system business.industry motor functional recovery Laminectomy medicine.disease Spinal cord spinal cord injury glutathione monoethyl ester neuroprotection neurorestoration peptides protective autoimmunity 030104 developmental biology medicine.anatomical_structure business 030217 neurology & neurosurgery Research Article |
| Zdroj: | Neural Regeneration Research Neural Regeneration Research, Vol 16, Iss 7, Pp 1273-1280 (2021) |
| ISSN: | 1876-7958 1673-5374 |
| Popis: | Several therapies have shown obvious effects on structural conservation contributing to motor functional recovery after spinal cord injury (SCI). Nevertheless, neither strategy has achieved a convincing effect. We purposed a combined therapy of immunomodulatory peptides that individually have shown significant effects on motor functional recovery in rats with SCI. The objective of this study was to investigate the effects of the combined therapy of monocyte locomotion inhibitor factor (MLIF), A91 peptide, and glutathione monoethyl ester (GSH-MEE) on chronic-stage spinal cord injury. Female Sprague-Dawley rats underwent a laminectomy of the T9 vertebra and a moderate contusion. Six groups were included: sham, PBS, MLIF + A91, MLIF + GSH-MEE, A91 + GSH-MEE, and MLIF + A91 + GSH-MEE. Two months after injury, motor functional recovery was evaluated using the open field test. Parenchyma and white matter preservation was evaluated using hematoxylin & eosin staining and Luxol Fast Blue staining, respectively. The number of motoneurons in the ventral horn and the number of axonal fibers were determined using hematoxylin & eosin staining and immunohistochemistry, respectively. Collagen deposition was evaluated using Masson's trichrome staining. The combined therapy of MLIF, A91, and GSH-MEE greatly contributed to motor functional recovery and preservation of the medullary parenchyma, white matter, motoneurons, and axonal fibres, and reduced the deposition of collagen in the lesioned area. The combined therapy of MLIF, A91, and GSH-MEE preserved spinal cord tissue integrity and promoted motor functional recovery of rats after SCI. This study was approved by the National Commission for Scientific Research on Bioethics and Biosafety of the Instituto Mexicano del Seguro Social under registration number R-2015-785-116 (approval date November 30, 2015) and R-2017-3603-33 (approval date June 5, 2017). |
| Databáze: | OpenAIRE |
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