Structure–activity relationships by mass spectrometry: identification of novel MMP-3 inhibitors
| Autor: | Kevin R Clark, Thomas R. Gadek, Martin E. Struble, James H. Bourell, Denise A. Ockey, Jenna Dotson, John T. Stults |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Stereochemistry
medicine.drug_class Clinical Biochemistry Pharmaceutical Science Carboxamide Matrix Metalloproteinase Inhibitors Biochemistry Mass Spectrometry Stromelysin 1 Structure-Activity Relationship chemistry.chemical_compound Drug Discovery medicine Protease Inhibitors Binding site Molecular Biology chemistry.chemical_classification Hydroxamic acid biology Chemistry Organic Chemistry Enzyme Enzyme inhibitor biology.protein Molecular Medicine Matrix Metalloproteinase 3 Target protein Function (biology) |
| Zdroj: | Bioorganic & Medicinal Chemistry. 12:37-44 |
| ISSN: | 0968-0896 |
| Popis: | A novel class of nonpeptide inhibitors of stromelysin (MMP-3) has been discovered with the use of mass spectrometry. The method relies on the development of structure–activity relationships by mass spectrometry (SAR by MS) and utilizes information derived from the binding of known inhibitors to identify novel inhibitors of a target protein with a minimum of synthetic effort. Noncovalent complexes of known inhibitors with a target protein are analyzed; these inhibitors are deconstructed into sets of fragments which compete for common or overlapping binding sites on the target protein. The binding of each fragment set can be studied independently. With the use of competition studies, novel members of each fragment set are identified from compound libraries that bind to the same site on the target protein. A novel inhibitor of the target protein was then constructed by chemically linking a combination of members of each fragment set in a manner guided by the proximity and orientation of the fragments derived from the known inhibitors. In the case of stromelysin, a novel inhibitor composed of favorably linked fragments was observed to form a 1:1 complex with stromelysin. Compounds that were not linked appropriately formed higher order complexes with stoichiometries of 2:1 or greater. These linked molecules were subsequently assessed for their ability to block stromelysin function in a chromogenic substrate assay. |
| Databáze: | OpenAIRE |
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