Protein-RNA interactions in the active center of transcription elongation complex
| Autor: | Arkady Mustaev, Alex Goldfarb, Vadim Markovtsov |
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| Rok vydání: | 1996 |
| Předmět: |
Transcription
Genetic Protein Conformation Molecular Sequence Data RNA-dependent RNA polymerase RNA polymerase II chemistry.chemical_compound Bacterial Proteins Transcription (biology) RNA polymerase Escherichia coli RNA polymerase I Amino Acid Sequence Binding Sites Multidisciplinary Sequence Homology Amino Acid biology General transcription factor Chromosome Mapping RNA Affinity Labels DNA-Directed RNA Polymerases Molecular biology Post-transcriptional modification Cell biology RNA Bacterial Cross-Linking Reagents chemistry Molecular Probes biology.protein Research Article |
| Zdroj: | Proceedings of the National Academy of Sciences. 93:3221-3226 |
| ISSN: | 1091-6490 0027-8424 |
| Popis: | By using a crosslinkable probe incorporated into the 3' terminus of nascent transcript, three sites were mapped in Escherichia coli RNA polymerase that are contacted by the RNA in the productive elongation complex. Two of these sites are in the beta subunit and one is in the beta' subunit. During elongation, the transcription complex occasionally undergoes an arrest whereby it can neither extend nor release the RNA transcript. It is demonstrated that in an arrested complex, the three contacts of RNA 3' terminus are lost, while a new beta' subunit contact becomes prominent. Thus, elongation arrest appears to involve the disengagement of the bulk of the active center from the 3' terminus of RNA and the transfer of the terminus into a new protein environment. |
| Databáze: | OpenAIRE |
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