Short poly(A) tails are a conserved feature of highly expressed genes
| Autor: | Ying Hsin Chen, Laura B. Chipman, Sarah Azoubel Lima, Gene W. Yeo, Jeff Coller, Amy E. Pasquinelli, Brian A. Yee, Angela L. Nicholson |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
RNA Untranslated Translational efficiency Peptide Chain Elongation Translational Gene Expression Translational Inhibition Ribosome 03 medical and health sciences Structural Biology Gene expression Animals RNA Messenger Caenorhabditis elegans Molecular Biology Gene Regulation of gene expression biology Chemistry RNA High-Throughput Nucleotide Sequencing biology.organism_classification Cell biology 030104 developmental biology Gene Expression Regulation Poly A |
| Zdroj: | Nature structuralmolecular biology. 24(12) |
| ISSN: | 1545-9985 |
| Popis: | Poly(A) tails are important elements in mRNA translation and stability, although recent genome-wide studies have concluded that poly(A) tail length is generally not associated with translational efficiency in nonembryonic cells. To investigate whether poly(A) tail size might be coupled to gene expression in an intact organism, we used an adapted TAIL-seq protocol to measure poly(A) tails in Caenorhabditis elegans. Surprisingly, we found that well-expressed transcripts contain relatively short, well-defined tails. This attribute appears to be dependent on translational efficiency, as transcripts enriched for optimal codons and ribosome association had the shortest tail sizes, whereas noncoding RNAs retained long tails. Across eukaryotes, short tails were a feature of abundant and well-translated mRNAs. This seems to contradict the dogma that deadenylation induces translational inhibition and mRNA decay and suggests that well-expressed mRNAs accumulate with pruned tails that accommodate a minimal number of poly(A)-binding proteins, which may be ideal for protective and translational functions. |
| Databáze: | OpenAIRE |
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