Mucus Microrheology Measured on Human Bronchial Epithelium Culture
| Autor: | Christophe Blanc, Charlotte Vernisse, Myriam Jory, Laura Casanellas, Arnaud Bourdin, Gladys Massiera, Isabelle Vachier, Karim Bellouma, Aurélie Petit, Paul Reynaud |
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| Přispěvatelé: | Laboratoire Charles Coulomb (L2C), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Physiologie & médecine expérimentale du Cœur et des Muscles [U 1046] (PhyMedExp), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), MORNET, Dominique, Université de Montpellier (UM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Microrheology
Materials Science (miscellaneous) human bronchial epithelium (HBE) [PHYS.PHYS.PHYS-BIO-PH]Physics [physics]/Physics [physics]/Biological Physics [physics.bio-ph] Biophysics General Physics and Astronomy 02 engineering and technology biopolymer gels 03 medical and health sciences fluids and secretions mucus medicine optical tweezers (OT) Physical and Theoretical Chemistry microrheology Mathematical Physics viscoelasticity 030304 developmental biology Collection methods 0303 health sciences [PHYS.PHYS.PHYS-BIO-PH] Physics [physics]/Physics [physics]/Biological Physics [physics.bio-ph] Chemistry Mucin mucins respiratory system 021001 nanoscience & nanotechnology Mucus Epithelium lcsh:QC1-999 [PHYS.COND.CM-SCM] Physics [physics]/Condensed Matter [cond-mat]/Soft Condensed Matter [cond-mat.soft] medicine.anatomical_structure rheology 0210 nano-technology Bronchial epithelium [PHYS.COND.CM-SCM]Physics [physics]/Condensed Matter [cond-mat]/Soft Condensed Matter [cond-mat.soft] lcsh:Physics |
| Zdroj: | Frontiers in Physics Frontiers in Physics, 2019, 7, pp.19. ⟨10.3389/fphy.2019.00019⟩ Frontiers in Physics, Frontiers, 2019, 7, pp.19. ⟨10.3389/fphy.2019.00019⟩ Frontiers in Physics, Vol 7 (2019) |
| ISSN: | 2296-424X |
| Popis: | We describe an original method to measure mucus microrheology on human bronchial epithelium culture using optical tweezers. We probed rheology on the whole thickness of mucus above the epithelium and showed that mucus gradually varies in rheological response, from an elastic behavior close to the epithelium to a viscous one far away. Microrheology was also performed on mucus collected on the culture, on ex vivo mucus collected by bronchoscopy, and on another epithelium model. Differences are discussed and are related to mucus heterogeneity, adhesiveness, and collection method. |
| Databáze: | OpenAIRE |
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