Whitefly feeding behavior and retention of a foregut-borne crinivirus exposed to artificial diets with different pH values

Autor: Angel Y.S. Chen, Jaclyn S Zhou, Alyssa Carpenter, Martin Drucker, Nicole H. Lopez, James C. K. Ng
Přispěvatelé: Department of Plant Pathology and Microbiology, and Center for Disease Vector Research, University of California [Riverside] (UCR), University of California-University of California, Biologie et Génétique des Interactions Plante-Parasite (UMR BGPI), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Barry University, East Stroudsburg University, Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Human Frontier Science Program : RGP0013/2015, National Science Foundation : 1146797, UC Riverside
Jazyk: angličtina
Rok vydání: 2017
Předmět:
Male
0301 basic medicine
retention
hemiptera
Whitefly
General Biochemistry
Genetics and Molecular Biology
Virus
crinivirus
03 medical and health sciences
Crinivirus
Animal science
Electrical penetration graph
noncirculative semipersistent transmission
transmission de virus
Transmission of plant viruses
whitefly
Animals
inoculation
pathologie végétale
Ecology
Evolution
Behavior and Systematics
biology
Inoculation
dietary pH
Foregut
Feeding Behavior
Hydrogen-Ion Concentration
biology.organism_classification
Virology
maladie des plantes
Diet
Insect Vectors
030104 developmental biology
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology
Insect Science
[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology
Female
mouche blanche
Agronomy and Crop Science
Lettuce infectious yellows virus
Zdroj: Insect Science
Insect Science, Wiley, 2017, pp.1-14. ⟨10.1111/1744-7917.12503⟩
Insect Science, Wiley, 2017, 24 (6), pp.1-14. ⟨10.1111/1744-7917.12503⟩
ISSN: 1672-9609
1744-7917
DOI: 10.1111/1744-7917.12503⟩
Popis: Transmission of plant viruses by phytophagous hemipteran insects encompasses complex interactions underlying a continuum of processes involved in virus acquisition, retention and inoculation combined with vector feeding behavior. Here, we investigated the effects of dietary pH on whitefly (Bemisia tabaci) feeding behavior and release of Lettuce infectious yellows virus (LIYV) virions retained in the vector's foregut. Electrical penetration graph analysis revealed that variables associated with whitefly probing and ingestion did not differ significantly in pH (4, 7.4, and 9)-adjusted artificial diets. To investigate virus retention and release, whiteflies allowed to acquire LIYV virions in a pH 7.4 artificial diet were fed pH 4, 7.4, or 9 virion-free artificial (clearing) diets. Immunofluorescent localization analyses indicated that virions remained bound to the foreguts of approximately 20%–24% of vectors after they fed on each of the three pH-adjusted clearing diets. When RNA preparations from the clearing diets were analyzed by reverse transcription (RT) nested-PCR and, in some cases, real-time qPCR, successful amplification of LIYV-specific sequence was infrequent but consistently repeatable for the pH 7.4 diet but never observed for the pH 4 and 9 diets, suggesting a weak pH-dependent effect for virion release. Viruliferous vectors that fed on each of the three pH-adjusted clearing diets transmitted LIYV to virus-free plants. These results suggest that changes in pH values alone in artificial diet do not result in observable changes in whitefly feeding behaviors, an observation that marks a first in the feeding of artificial diet by whitefly vectors; and that there is a potential causal and contingent relationship between the pH in artificial diet and the release/inoculation of foregut bound virions. This article is protected by copyright. All rights reserved
Databáze: OpenAIRE
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