Mitogen-activated protein kinase 4-like carrying an MEY motif instead of a TXY motif is involved in ozone tolerance and regulation of stomatal closure in tobacco
Autor: | Hiroshi Yoda, Kazuyuki Kuchitsu, Mitsuko Aono, Yuki Yanagawa, Ichiro Mitsuhara, Kohei Osaki, Yuta Amano, Shigemi Seo |
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Jazyk: | angličtina |
Rok vydání: | 2016 |
Předmět: |
0106 biological sciences
0301 basic medicine MAPK/ERK pathway Nicotiana MPK4 Physiology Nicotiana tabacum stomata Plant Science 01 natural sciences 03 medical and health sciences Gene Expression Regulation Plant Tobacco Amino Acid Sequence Tyrosine tabacum Protein kinase A Phylogeny Plant Proteins biology integumentary system Kinase fungi TEY motif biology.organism_classification ozone 030104 developmental biology Biochemistry Mitogen-activated protein kinase Plant Stomata biology.protein MAP kinase Signal transduction Mitogen-Activated Protein Kinases 010606 plant biology & botany Research Paper |
Zdroj: | Journal of Experimental Botany SC30201706190008 |
ISSN: | 1460-2431 0022-0957 |
DOI: | 10.1093/jxb/erw173 |
Popis: | Highlight Tobacco MPK4L is activated by wounding and ozone exposure in a pathway independent to that of MPK4 and plays a role in the control of stomatal aperture. The mitogen-activated protein kinases (MAPKs/MPKs) are important factors in the regulation of signal transduction in response to biotic and abiotic stresses. Previously, we characterized a MAPK from tobacco, Nicotiana tabacum MPK4 (NtMPK4). Here, we found a highly homologous gene, NtMPK4-like (NtMPK4L), in tobacco as well as other species in Solanaceae and Gramineae. Deduced amino acid sequences of their translation products carried MEY motifs instead of conserved TXY motifs of the MAPK family. We isolated the full length NtMPK4L gene and examined the physiological functions of NtMPK4L. We revealed that NtMPK4L was activated by wounding, like NtMPK4. However, a constitutively active salicylic acid-induced protein kinase kinase (SIPKKEE), which phosphorylates NtMPK4, did not phosphorylate NtMPK4L. Moreover, a tyrosine residue in the MEY motif was not involved in NtMPK4L activation. We also found that NtMPK4L-silenced plants showed rapid transpiration caused by remarkably open stomata. In addition, NtMPK4L-silenced plants completely lost the ability to close stomata upon ozone treatment and were highly sensitive to ozone, suggesting that this atypical MAPK plays a role in ozone tolerance through stomatal regulation. |
Databáze: | OpenAIRE |
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