Successful transportation of human corneal endothelial tissues without cool preservation in varying Indian tropical climatic conditions and in vitro cell expansion using a novel polymer

Autor: Rajappa Senthilkumar, Subramani Baskar, Shigeo Tsukahara, Hiroshi Yoshioka, Thangavelu Srinivasan, John Sudhakar, Samuel J. K. Abraham, Yuichi Mori, Aditya Insaan, Periyasamy Parikumar, Paramasivam Thamaraikannan, Senthilkumar Preethy, Srinivas K Rao, Sadananda Rao Manjunath, Sundaram Natarajan
Rok vydání: 2014
Předmět:
Polymers
medicine.medical_treatment
Cell
Blindness
Low vision care
in vitro expansion
supraciliary segment implants
Corneal Transplantation
lcsh:Ophthalmology
human corneal endothelial precursor cells
Cornea
risk factors
Aged
80 and over
patching
Endothelium
Corneal
Middle Aged
reading performance
Tissue Donors
medicine.anatomical_structure
thermo-reversible gelation polymer
cataract
Original Article
Tissue Preservation
eye lens
Adult
Accommodation
Corneal endothelium
medicine.medical_specialty
Adolescent
Endothelium
India
In Vitro Techniques
Amblyopia
Andrology
Young Adult
Cadaver
treatment of cataract
medicine
Humans
multiple disabilities and visual impairment
Viability assay
Corneal transplantation
Aged
transportation
Tropical Climate
business.industry
citicoline
In vitro
Surgery
Transplantation
Ophthalmology
lcsh:RE1-994
presbyopia
sphere forming assay
business
Zdroj: Indian Journal of Ophthalmology, Vol 62, Iss 2, Pp 130-135 (2014)
Indian Journal of Ophthalmology
ISSN: 0301-4738
DOI: 10.4103/0301-4738.116457
Popis: Background: Though the transplantation of human corneal endothelial tissue (CET) separated from cadaver cornea is in practice, its transportation has not been reported. We report the successful transportation of CET in varying Indian climatic conditions without cool preservation and the in vitro expansion of Human Corneal Endothelial Precursor Cells (HCEPCs) using a novel Thermo-reversible gelation polymer (TGP). Materials and Methods: CET from cadaver corneas (n = 67), unsuitable for transplantation, were used. In phase I, CET was transported in Basal Culture Medium (Group I) and TGP (Group II) and in Phase II, in TGP cocktail alone, from three hospitals 250-2500 km away, to a central laboratory. The transportation time ranged from 6 h to 72 h and the outdoor temperature between 20°C and 41°C. On arrival, CET were processed, cells were expanded upto 30 days in basal culture medium (Group A) and TGP scaffold (Group B). Cell viability and morphology were documented and Reverse transcription polymerase chain reaction (RT-PCR) characterization undertaken. Results: In Phase I, TGP yielded more viable cells (0.11 × 10 6 cells) than Group I (0.04 × 10 6 cells). In Phase II, the average cell count was 5.44 × 10 4 cells. During expansion, viability of HCEPCs spheres in TGP was maintained for a longer duration. The cells from both the groups tested positive for B-3 tubulin and negative for cytokeratins K3 and K12, thereby proving them to be HCEPCs. Conclusion: TGP preserves the CET during transportation without cool preservation and supports in vitro expansion, with a higher yield of HCEPCs, similar to that reported in clinical studies.
Databáze: OpenAIRE
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