Isolation and characterization of a microspore-specific gene from tobacco
| Autor: | J. H. Visser, P. F. M. de Groot, George J. Wullems, J.A.M. Schrauwen, Margryt T. Oldenhof |
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| Rok vydání: | 1996 |
| Předmět: |
clone (Java method)
DNA Complementary Nicotiana tabacum Molecular Sequence Data Plant Science Biology Genes Plant Gametogenesis Microspore Complementary DNA Tobacco Genetics Gene family Amino Acid Sequence RNA Messenger Cloning Molecular GeneralLiterature_REFERENCE(e.g. dictionaries encyclopedias glossaries) Gene Plant Proteins Southern blot Base Sequence cDNA library Gene Expression Regulation Developmental food and beverages General Medicine biology.organism_classification Molecular biology Plants Toxic Solubility Agronomy and Crop Science |
| Zdroj: | Plant Molecular Biology, 31, 2, pp. 213-225 Plant Molecular Biology, 31, 213-225. Kluwer academic publ |
| ISSN: | 1573-5028 0167-4412 |
| DOI: | 10.1007/bf00021785 |
| Popis: | The characterization of a gene with a unique microspore-specific expression pattern is reported. Isolated microspores from tobacco were used to synthesize a cDNA library. Clones that did not hybridize to leaf cDNA were further characterized by northern analysis. One clone proved to be a microspore-specific cDNA, representing a transcript of 650 nt. The corresponding gene, NTM19 (Nicotiana tabacum microspore-specific), was isolated and its sequence analysed. The gene encodes a protein of 10.8 kDa with a pI of 6.92 and a putative signal sequence at the N-terminus. A localization study revealed a unique spatial and temporal distribution. The transcript was only detected in the unicellular microspore. No hybridization signals were observed in other pollen developmental stages, nor in the surrounding anther tissues or other vegetative tissues of the plant. Therefore it can be concluded that NTM19 is a gene with a highly microspore-specific character according to both localization and stage of expression. Southern blot analysis demonstrated the presence of a small gene family. The occurrence of TNM19 was investigated in a range of closely and distantly related species and was found to be present in other solanaceous species, including the ancestors of tobacco and in a monocot species. |
| Databáze: | OpenAIRE |
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