A calcium-protease activator associated with brain microsomal-insoluble elements
| Autor: | Yuko Uratsuji, Akira Kishimoto, Hiroyuki Nakanishi, Yasutomi Nishizuka, Y Takeyama |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
Calmodulin
medicine.medical_treatment Biophysics chemistry.chemical_element Calcium Biochemistry Structural Biology Microsomes Casein Centrifugation Density Gradient Genetics medicine Animals Molecular Biology Calpain Calpain activator Protease Brain Chemistry Protease biology Calpain Chemistry Hydrolysis Caseins Proteins Cell Biology Sucrose gradient Enzyme Activation Solubility Microsome biology.protein Cattle Calpain II |
| Zdroj: | FEBS Letters. (1):110-114 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/0014-5793(86)80060-6 |
| Popis: | A factor which markedly activates Ca 2+ -dependent thiol protease (calpain) is associated with Triton X-100-insoluble materials, presumably structural elements such as cytoskeletons, of bovine brain microsomal fraction. This factor is extracted with 0.6 M KCl, and purified partially by sucrose density gradient centrifugation and hydroxyapatite column chromatography. The factor appears to be a heat-stable protein with an approximate M r , of 15000. With casein as substrate this factor activates both calpain I and calpain II severalfold up to more than 10- fold without alteration of their affinity to Ca 2+ . Calmodulin is unable to substitute for this factor. A similar factor is associated with human platelet insoluble materials. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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