Singlet oxygen-mediated one-pot chemoselective peptide–peptide ligation
| Autor: | Eirini Antonatou, Annemieke Madder, Yentl Verleysen |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
chemistry.chemical_classification
Singlet Oxygen 010405 organic chemistry Singlet oxygen Organic Chemistry Hydrazone Peptide 010402 general chemistry Hydrazide Native chemical ligation 01 natural sciences Biochemistry Combinatorial chemistry 0104 chemical sciences chemistry.chemical_compound chemistry Furan Click chemistry Organic chemistry Amino Acid Sequence Chemical ligation Physical and Theoretical Chemistry Peptides Protein Binding |
| Zdroj: | Organic & Biomolecular Chemistry. 15:8140-8144 |
| ISSN: | 1477-0539 1477-0520 |
| DOI: | 10.1039/c7ob02245j |
| Popis: | We here describe a furan oxidation based site-specific chemical ligation approach using unprotected peptide segments. This approach involves two steps: after photooxidation of a furan-containing peptide, ligation is achieved by reaction of the unmasked keto-enal with C- or N-terminal α-nucleophilic moieties of the second peptide such as hydrazine or hydrazide to form a pyridazinium or pyrrolidinone linkage respectively. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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