Metabolic versatility of freshwater sedimentary archaea feeding on different organic carbon sources

Autor: Frederic Gich, Mireia Fillol, Carles M. Borrego, Sergi Compte-Port
Jazyk: angličtina
Rok vydání: 2020
Předmět:
cDNA libraries
Geologic Sediments
Marine and Aquatic Sciences
Biochemistry
RNA
Ribosomal
16S
DNA libraries
Archaean Biology
Phylogeny
Sedimentary Geology
Total organic carbon
0303 health sciences
Multidisciplinary
biology
Chemistry
Tryptophan
Geology
Biodiversity
Plant litter
Complementary DNA
Nucleic acids
DNA
Archaeal
Ribosomal RNA
Medicine
Microcosm
Research Article
Freshwater Environments
Cell biology
Cellular structures and organelles
Forms of DNA
Science
Thermoplasmata
chemistry.chemical_element
Euryarchaeota
Microbiology
Carbon Cycle
Carbon cycle
03 medical and health sciences
Extraction techniques
Botany
Genetics
Non-coding RNA
Ecosystem
Humic Substances
Petrology
030304 developmental biology
030306 microbiology
Ecology and Environmental Sciences
Organisms
Crenarchaeota
Biology and Life Sciences
Aquatic Environments
Sequence Analysis
DNA
DNA
Bodies of Water
biology.organism_classification
Archaea
Carbon
RNA extraction
Research and analysis methods
Lakes
Biofilms
Earth Sciences
RNA
Sediment
Ribosomes
Zdroj: PLoS ONE, Vol 15, Iss 4, p e0231238 (2020)
PLoS ONE
ISSN: 1932-6203
Popis: Members of the phylum Bathyarchaeota and the class Thermoplasmata are widespread in marine and freshwater sediments where they have been recognized as key players in the carbon cycle. Here, we tested the responsiveness of archaeal communities on settled plant debris and sediment from a karstic lake to different organic carbon amendments (amino acids, plant-derived carbohydrates, and aromatics) using a lab-scale microcosm. Changes in the composition and abundance of sediment and biofilm archaeal communities in both DNA and RNA fractions were assessed by 16S rRNA gene amplicon sequencing and qPCR, respectively, after 7 and 30 days of incubation. Archaeal communities showed compositional changes in terms of alpha and beta diversity in relation to the type of carbon source (amino acids vs. plant-derived compounds), the nucleic acid fraction (DNA vs. RNA), and the incubation time (7 vs. 30 days). Distinct groups within the Bathyarchaeota (Bathy-15 and Bathy-6) and the Thermoplasmata (MBG-D) differently reacted to carbon supplements as deduced from the analysis of RNA libraries. Whereas Bathyarchaeota in biofilms showed a long-term positive response to humic acids, their counterparts in the sediment were mainly stimulated by the addition of tryptophan, suggesting the presence of different subpopulations in both habitats. Overall, our work presents an in vitro assessment of the versatility of archaea inhabiting freshwater sediments towards organic carbon and introduces settled leaf litter as a new habitat for the Bathyarchaeota and the Thermoplasmata.
Databáze: OpenAIRE
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