A Study of Opiate, Opiate Metabolites and Antihistamines in Urine after Consumption of Cold Syrups by LC-MS/MS
| Autor: | San-Chong Chyueh, Yao-Te Yen, Ting-Yueh Chen, Chi-Lun Chang, Yin-Jue Chang, Pin-Jung Lai |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Adult
Male Pyridines Metabolite Histamine Antagonists Pharmaceutical Science cold syrups Urine carbinoxamine 01 natural sciences Gas Chromatography-Mass Spectrometry Article Analytical Chemistry lcsh:QD241-441 Young Adult 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine lcsh:Organic chemistry Drug Discovery Lc ms ms medicine Humans 030216 legal & forensic medicine Physical and Theoretical Chemistry Derivatization codeine Morphine Derivatives Chromatography Opiate Alkaloids 010401 analytical chemistry Organic Chemistry Codeine morphine Forensic Medicine Middle Aged chlorpheniramine 0104 chemical sciences Analgesics Opioid chemistry Chemistry (miscellaneous) Morphine Molecular Medicine Carbinoxamine Female Opiate heroin medicine.drug |
| Zdroj: | Molecules, Vol 25, Iss 4, p 972 (2020) Molecules Volume 25 Issue 4 |
| ISSN: | 1420-3049 |
| Popis: | Studying the origin of opiate and/or opiate metabolites in individual urine specimens after consumption of cold syrups is vital for patients, doctors, and law enforcement. A rapid liquid chromatography&ndash tandem mass spectrometry method using &ldquo dilute-and-shoot&rdquo analysis without the need for extraction, hydrolysis and/or derivatization has been developed and validated. The approach provides linear ranges of 2.5&ndash 1000 ng mL&minus 1 for 6-acetylmorphine, codeine, chlorpheniramine, and carbinoxamine, 2.5&ndash 800 ng mL&minus 1 for morphine and morphine-3-&beta d-glucuronide, and 2.5&ndash 600 ng mL&minus 1 for morphine-6-&beta d-glucuronide and codeine-6-&beta d-glucuronide, with excellent correlation coefficients (R2 > 0.995) and matrix effects (< 5%). Urine samples collected from the ten participants orally administered cold syrups were analyzed. The results concluded that participants consuming codeine-containing cold syrups did not routinely pass urine tests for opiates, and their morphine&ndash codeine concentration ratios (M/C) were not always < 1. In addition, the distribution map of the clinical total concentration of the sum of morphine and codeine against the antihistamines (chlorpheniramine or carbinoxamine) were plotted for discrimination of people who used cold syrups. The 15 real cases have been studied by using M/C rule, cutoff value, and distribution map, further revealing a potential approach to determine opiate metabolite in urine originating from cold syrups. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|