Immunotoxic effects of smokeless tobacco on the accessory cell function of rat oral epithelium
| Autor: | Mats Wallström, Bengt Hasséus, Bengt-Göran Österdahl, Mats Jontell, Jan-Michael Hirsch |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Nicotine
Nitrosamines Tobacco Smokeless T-Lymphocytes Antigen-Presenting Cells Pharmacology Lymphocyte Activation Anabasine Epithelium Immune system Alkaloids medicine Concanavalin A Animals Oral mucosa Antigen-presenting cell General Dentistry IC50 Immunosuppressive effect business.industry Plant Extracts Mouth Mucosa Epithelial Cells Rats Plants Toxic medicine.anatomical_structure Smokeless tobacco Rats Inbred Lew Langerhans Cells Immunology Oral epithelium Carcinogens Mitogens Plant Lectins business Function (biology) Cell Division Immunosuppressive Agents Spleen Nitroso Compounds |
| Zdroj: | European journal of oral sciences. 105(1) |
| ISSN: | 0909-8836 |
| Popis: | Smokeless tobacco (ST) is known to adversely effect the oral mucosa, but knowledge about the influence on immune defence is limited. Few studies have investigated the effect of ST on the local immune response. In the present study, we have assessed the effect of a crude Swedish moist snuff (SS) extract, alkaloids, and nitrosamines on T-cell mitogenic response to Con A using epithelial cells, including Langerhans cells, of the rat oral mucosa as accessory cells. SS extract at a concentration of 4% reduced the T-cell proliferation by 50% (IC50 = 4%). Pretreatment of either oral epithelial cells or T-cells with SS extract also gave a significant inhibition of T-cell proliferation. This effect was not obtained following preincubation with SS components as alkaloids and different tobacco-specific nitrosamines (TSNA). None of the tested compounds were found to possess any mitogenic properties. This in vitro study showed that SS extract can evoke an immunosuppressive effect on mitogen-driven T-cell proliferation using cells from oral epithelium as accessory cells. This effect was more pronounced when SS extract was employed compared to when the single SS components were used alone. |
| Databáze: | OpenAIRE |
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