Genome-wide identification of novel ovarian-predominant miRNAs: new insights from the medaka (Oryzias latipes)
| Autor: | Amine Bouchareb, Aurélie Le Cam, Jérôme Montfort, Stéphanie Gay, Thaovi Nguyen, Julien Bobe, Violette Thermes |
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| Přispěvatelé: | Laboratoire de Physiologie et Génomique des Poissons (LPGP), Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )-Institut National de la Recherche Agronomique (INRA), ANR-13-BSV7-0015,Maternal Legacy,Portait moléculaire d'un oeuf de poisson de bonne qualité(2013), ANR-13-BSV7-0015-Maternal Legacy, Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ) |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
In silico Oryzias [SDV]Life Sciences [q-bio] Oogenesis Genome Article reproduction 03 medical and health sciences poisson microRNA Animals adrianichthyidae développement précoce Gene miRNA Regulation of gene expression Genetics fish medaka Multidisciplinary biology Microarray analysis techniques Ovary Gene Expression Regulation Developmental biology.organism_classification Microarray Analysis ovogenèse oryzias latipes MicroRNAs 030104 developmental biology Female expression des gènes |
| Zdroj: | Scientific Reports Scientific Reports, Nature Publishing Group, 2017, 7 (1), pp.1-11. ⟨10.1038/srep40241⟩ Scientific Reports, 1-11. (2017) |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/srep40241⟩ |
| Popis: | MicroRNAs (miRNAs) are small, highly conserved non-coding RNAs that play important roles in the regulation of many physiological processes. However, the role of miRNAs in vertebrate oocyte formation (i.e., oogenesis) remains poorly investigated. To gain new insights into the roles of miRNAs in oogenesis, we searched for ovarian-predominant miRNAs. Using a microarray displaying 3,800 distinct miRNAs originating from different vertebrate species, we identified 66 miRNAs that are expressed predominantly in the ovary. Of the miRNAs exhibiting the highest overabundance in the ovary, 20 were selected for further analysis. Using a combination of QPCR and in silico analyses, we identified 8 novel miRNAs that are predominantly expressed in the ovary, including 2 miRNAs (miR-4785 and miR-6352) that exhibit strict ovarian expression. Of these 8 miRNAs, 7 were previously uncharacterized in fish. The strict ovarian expression of miR-4785 and miR-6352 suggests an important role in oogenesis and/or early development, possibly involving a maternal effect. Together, these results indicate that, similar to protein-coding genes, a significant number of ovarian-predominant miRNA genes are found in fish. |
| Databáze: | OpenAIRE |
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