An experimental study on cervix cancer with combination of HSV-TK/GCV suicide gene therapy system and 60Co radiotherapy
| Autor: | Qiusha Tang, Daozhen Chen |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
Ganciclovir
Pathology medicine.medical_specialty Cancer Research medicine.medical_treatment Genetic enhancement viruses Mice Nude Uterine Cervical Neoplasms Antiviral Agents Thymidine Kinase lcsh:RC254-282 HeLa Mice In vivo Genetics Medicine Animals Humans Cobalt Radioisotopes Cervical cancer biology business.industry Genes Transgenic Suicide Cancer Genetic Therapy Suicide gene medicine.disease biology.organism_classification Prognosis lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens Combined Modality Therapy Radiation therapy Disease Models Animal Retroviridae Oncology Cancer research Female business medicine.drug HeLa Cells Research Article |
| Zdroj: | BMC Cancer, Vol 10, Iss 1, p 609 (2010) BMC Cancer |
| ISSN: | 1471-2407 |
| Popis: | Background To evaluate the killing effect of HSV-TK/GCV suicide gene therapy system combined with 60Co radiotherapy on human cervical cancer Hela cell line in vitro and in vivo, and to explore the radiosensitization by HSV-TK/GCV system. Methods HSV-TK/GCV suicide gene therapy system and 60Co radiotherapy were used separately or in combination on human cervical cancer Hela cell line in vitro and in vivo to compare their effects. Colony formation test and the rate of radiosensitization effect (E/O) were employed to observed the radiosensitization by HSV-TK/GCV system. Results HSV-TK/GCV suicide gene therapy system had strong therapeutic effects on Hela cells in vitro and in vivo (the inhibition rates were 45.8% and 39.5%, respectively), moreover, the combined administration of gene therapy and radiotherapy had stronger therapeutic effects in vitro and in vivo (the inhibition rate was 87.5% in vitro, and the inhibition rate was 87.9% in vivo) (P < 0.01). The inhibition rate by radiotherapy alone was 42.4% in vitro and 35.8% in vivo. The sensitivity of combined therapy to radiotherapy increased more than that of therapy alone, the ability of colony formation decreased (P < 0.01). The rate of radiosensitivity effect (E/O) was 3.17(> 1.4), indicating HSV-TK/GCV system could exert a sensitizing effect on 60Co radiotherapy of the transplanted human cervical cancer cell in nude mice. Conclusion HSV-TK/GCV system had radiosensitization. Gene therapy combined with radiotherapy may be a good supplementary method for cervix cancer synthetic treatment. |
| Databáze: | OpenAIRE |
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