Crystallization and preliminary data analysis of flock house virus
| Autor: | J. P. Wery, John E. Johnson, B. R. McKinney, Andrew J. Fisher |
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| Rok vydání: | 1992 |
| Předmět: |
Chemical Phenomena
Chemistry Physical Chemistry Resolution (electron density) Mineralogy Insect Viruses Flock house virus General Medicine Crystal structure General Biochemistry Genetics and Molecular Biology law.invention Crystal Viral Proteins Crystallography X-Ray Diffraction law Group (periodic table) X-ray crystallography Animals RNA Viruses Drosophila Orthorhombic crystal system Crystallization |
| Zdroj: | Acta Crystallographica Section B Structural Science. 48:515-520 |
| ISSN: | 0108-7681 |
| Popis: | Flock House virus, purified from infected cultured Drosophila cells, crystallizes into three different forms under identical growth conditions. Two crystal forms grow in the trigonal space group R3, both with equivalent cell constants a = 323.6 A, alpha = 61.7 degrees. The difference between the two trigonal crystal forms is 1.1 degrees in the orientation of the virus particle as determined from the rotation function. Early crystal setups grew in one form, while recent crystals grew in the other form. The third space group, which accounts for 5% of the observed crystals and grows with both trigonal forms, is orthorhombic I222 with cell parameters a = 416.7, b = 332.1, c = 351.2 A. The trigonal crystal forms contain one virion per unit cell and the orthorhombic form contains two particles per cell. All three crystal forms diffract X-rays to 2.8 A resolution. |
| Databáze: | OpenAIRE |
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