Setaria viridis as a model for translational genetic studies of jasmonic acid-related insect defenses in Zea mays
| Autor: | Qin-Bao Li, Charles T. Hunter, Anna Block, Caitlin C. Rering, Hans T. Alborn, Shawn A. Christensen |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Setaria Insecta Setaria Plant Cyclopentanes Plant Science Zea mays 01 natural sciences 03 medical and health sciences chemistry.chemical_compound Genetics Animals Gene family Herbivory Oxylipins Secondary metabolism Gene Plant Proteins biology Phylogenetic tree Setaria viridis Jasmonic acid General Medicine biology.organism_classification 030104 developmental biology chemistry Protein Biosynthesis Agronomy and Crop Science Orthologous Gene 010606 plant biology & botany |
| Zdroj: | Plant Science. 291:110329 |
| ISSN: | 0168-9452 |
| Popis: | Little is known regarding insect defense pathways in Setaria viridis (setaria), a model system for panicoid grasses, including Zea mays (maize). It is thus of interest to compare insect herbivory responses of setaria and maize. Here we use metabolic, phylogenetic, and gene expression analyses to measure a subset of jasmonic acid (JA)-related defense responses to leaf-chewing caterpillars. Phylogenetic comparisons of known defense-related maize genes were used to identify putative orthologs in setaria, and candidates were tested by quantitative PCR to determine transcriptional responses to insect challenge. Our findings show that while much of the core JA-related metabolic and genetic responses appear conserved between setaria and maize, production of downstream secondary metabolites such as benzoxazinoids and herbivore-induced plant volatiles are dissimilar. This diversity of chemical defenses and gene families involved in secondary metabolism among grasses presents new opportunities for cross species engineering. The high degree of genetic similarity and ease of orthologous gene identification between setaria and maize make setaria an excellent species for translational genetic studies, but the species specificity of downstream insect defense chemistry makes some pathways unamenable to cross-species comparisons. |
| Databáze: | OpenAIRE |
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