A new rabbit model of impaired wound healing in an X-ray-irradiated field

Autor: Kenichiro Kawai, Soh Nishimoto, Yohei Sotsuka, Toshihiro Fujiwara, Maki Tonooka, Masao Kakibuchi, Kazutoshi Fujita
Rok vydání: 2017
Předmět:
Pathology
Physiology
Angiogenesis
Dermatologic Surgical Procedures
lcsh:Medicine
Biochemistry
030207 dermatology & venereal diseases
0302 clinical medicine
Bone Marrow
Animal Cells
Immune Physiology
Group-Specific Staining
Medicine and Health Sciences
Platelet
lcsh:Science
Skin
Staining
Mammals
Multidisciplinary
integumentary system
X-ray
Animal Models
Body Fluids
Blood
medicine.anatomical_structure
Experimental Organism Systems
Cell Tracking
030220 oncology & carcinogenesis
Vertebrates
Female
Rabbits
Anatomy
Cellular Types
Research Article
Platelets
medicine.medical_specialty
Histology
Imaging Techniques
Immunology
Image Analysis
Research and Analysis Methods
Radiation Dosage
03 medical and health sciences
Tissue Repair
medicine
Animals
Irradiation
Wound Healing
Blood Cells
business.industry
X-Rays
Hematoxylin Staining
lcsh:R
Organisms
Biology and Life Sciences
Proteins
Cell Biology
Disease Models
Animal
Specimen Preparation and Treatment
Immune System
Amniotes
Rabbit model
lcsh:Q
Bone marrow
Physiological Processes
business
Wound healing
Collagens
Zdroj: PLoS ONE, Vol 12, Iss 9, p e0184534 (2017)
PLoS ONE
ISSN: 1932-6203
Popis: Radiation is an important therapy for cancer with many benefits; however, its side effects, such as impaired wound healing, are a major problem. While many attempts have been made to overcome this particular disadvantage, there are few effective treatments for impaired wound healing in an X-ray-irradiated field. One reason for this deficiency is the lack of experimental models, especially animal models. We have previously reported a mouse model of impaired wound healing in which the irradiation area was restricted to the hindlimbs. In this mouse model, due to the size of the animal, a diameter of five millimeters was considered the largest wound size suitable for the model. In addition, the transplanted cells had to be harvested from other inbred animals. To investigate larger wounds and the impact of autologous specimen delivery, a rabbit model was developed. Rabbits were kept in a special apparatus to shield the body and hindlimbs while the irradiation field was exposed to radiation. Six weeks after irradiation, a 2 x 2 cm, full-thickness skin defect was made inside the irradiation field. Then, the wound area was observed over time. The wound area after irradiation was larger than that without irradiation at all time points. Both angiogenesis and collagen formation were reduced. For further study, as an example of using this model, the effect of autologous platelet-rich plasma (PRP) was observed. Autologous PRP from peripheral blood (pb-PRP) and bone marrow aspirate (bm-PRP) was processed and injected into the wounds in the irradiated field. Two weeks later, the wounds treated with bm-PRP were significantly smaller than those treated with phosphate buffer vehicle controls. In contrast, the wounds treated with pb-PRP were not significantly different from the controls. This rabbit model is useful for investigating the mechanism of impaired wound healing in an X-ray-irradiated field.
Databáze: OpenAIRE
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