A three-step approach identifies novel shear stress-sensitive endothelial microRNAs involved in vasculoprotective effects of high-intensity interval training (HIIT)
| Autor: | Hans-Joachim Schnittler, Florian Rolfes, Michael Krüger, Bothaynah Jubran, Lothar Thorwesten, Franziska L. Breulmann, Boris Schmitz, Andreas Klose, Stefan-Martin Brand |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Endothelium endothelium business.industry Physical exercise Pharmacology Interval training Umbilical vein shear stress high-intensity training (HIT) 03 medical and health sciences 030104 developmental biology 0302 clinical medicine medicine.anatomical_structure Oncology In vivo 030220 oncology & carcinogenesis Circulatory system microRNA medicine business High-intensity interval training performance Research Paper miRNA |
| Zdroj: | Oncotarget |
| ISSN: | 1949-2553 |
| Popis: | Circulatory microRNAs (c-miRNAs) are regulated in response to physical activity and may exert anti-atherosclerotic effects. Since the vascular endothelium is an abundant source of c-miRNAs, we aimed to identify novel vasculoprotective exercise-induced c-miRNAs by the combined analysis of published endothelial miRNA array data followed by in vivo and in vitro validation. We identified 8 different array-based publications reporting 185 endothelial shear stress-regulated miRNAs of which 13 were identified in ≥3 independent reports. Nine miRNAs had already been associated with physical activity. Of the remaining novel miRNAs, miR-98-3p and miR-125-5p were selected for further analysis due to reported vasculoprotective effects. Analysis in two different 4-week high-intensity interval training (HIIT) groups (group 1 [n=27]: 4x30 s, group 2 [n=25]: 8x15 s; all-out running) suggested significantly elevated miR-98 and miR-125a-5p levels in response to acute exercise at baseline and at follow-up. Endothelial in vitro shear stress experiments revealed increased miR-125a-5p and miR-98-3p levels in medium of human umbilical vein endothelial cells at 30 dyn/cm2 after 20 and 60 min, respectively. Our results suggest that miR-98-3p and miR-125a-5p can be rapidly secreted by endothelial cells, which might be the source of increased c-miR-98-3p and -125a-5p levels in response to HIIT. Both miRNAs attenuate endothelial inflammation and may mediate vasculoprotective effects of physical exercise including HIIT. |
| Databáze: | OpenAIRE |
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