p-ERK1/2 is a predictive factor of response to erythropoiesis-stimulating agents in low/int-1 myelodysplastic syndromes
| Autor: | Patrycja Pawlikowska, Sophie Park, Vivian Viallon, Cecile Pierre-Eugene, Laure Gibault, Patrick Mayeux, Emilie Frisan, Françoise Porteu, François Dreyfus, Michaela Fontenay |
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| Rok vydání: | 2010 |
| Předmět: |
Male
Oncology medicine.medical_specialty Erythroblasts Biology Gene Expression Regulation Enzymologic Flow cytometry Internal medicine medicine Humans Erythropoiesis Erythropoietin Cells Cultured Aged Cell Proliferation Aged 80 and over Mitogen-Activated Protein Kinase 1 Mitogen-Activated Protein Kinase 3 Hematology medicine.diagnostic_test Myelodysplastic syndromes Flow Cytometry medicine.disease Red blood cell medicine.anatomical_structure Endocrinology Myelodysplastic Syndromes Hematinics Biomarker (medicine) Brief Reports Female Bone marrow Biomarkers medicine.drug |
| Zdroj: | Haematologica. 95:1964-1968 |
| ISSN: | 1592-8721 0390-6078 |
| DOI: | 10.3324/haematol.2010.024349 |
| Popis: | Serum erythropoietin level less than 100U/L and a transfusion requirement of less than 2 units per month are the best predictive factors for response to treatment by erythropoiesis-stimulating agents in low/int-1 myelodysplastic syndromes. To investigate the factors influencing the response to erythropoiesis-stimulating agents, we enrolled 127 low/int-1 myelodysplastic syndrome patients at diagnosis in a biological study of erythropoiesis. The 54 non-responders had a significantly lower number of burst-forming unit-erythroid and colony-forming unit-erythroid than responders. Erythropoietin-dependent proliferation and survival, and phospho (p)-ERK1/2 expression in steady state and after erythropoietin stimulation were defective in cultured erythroblasts. By flow cytometry, p-ERK1/2 was significantly lower in bone marrow CD45(-)/CD71(+)/GPA(-)cells from non-responders compared to responders or controls. Receiver Operator Characteristic curve analysis showed that this flow cytometry test was a sensitive biomarker for predicting the response to erythropoiesis-stimulating agents. |
| Databáze: | OpenAIRE |
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