Use of Oligoarrays for Characterization of Community-Onset Methicillin-Resistant Staphylococcus aureus

Autor: Daniel Pablo Lew, Antoine Huyghe, Stéphan Juergen Harbarth, Sasi Dharan, Yvan Charbonnier, Thibaud Koessler, Manuela Bento, Didier Pittet, Gesuele Renzi, Patrice Francois, Jacques Schrenzel
Rok vydání: 2006
Předmět:
Oligonucleotide Array Sequence Analysis/ methods
Adult
DNA
Bacterial
Male
Microbiology (medical)
Staphylococcus aureus
Cross Infection/epidemiology/microbiology
Minisatellite Repeats
Biology
medicine.disease_cause
Staphylococcal infections
Genome
Microbiology
Switzerland/epidemiology
medicine
Humans
Typing
Child
Phylogeny
Aged
Oligonucleotide Array Sequence Analysis
ddc:616
Genetics
Cross Infection
Molecular Epidemiology
Base Sequence
Molecular epidemiology
Community-Acquired Infections/epidemiology/ microbiology
Methicillin Resistance/ genetics
Bacteriology
Middle Aged
Staphylococcal Infections
Staphylococcus aureus/ drug effects/ genetics/isolation & purification
medicine.disease
Methicillin-resistant Staphylococcus aureus
Electrophoresis
Gel
Pulsed-Field
Community-Acquired Infections
DNA
Bacterial/genetics
Variable number tandem repeat
Staphylococcal Infections/epidemiology/ microbiology
Female
Methicillin Resistance
DNA microarray
Genome
Bacterial
Switzerland
Zdroj: Journal of Clinical Microbiology, Vol. 44, No 3 (2006) pp. 1040-1048
ISSN: 1098-660X
0095-1137
DOI: 10.1128/jcm.44.3.1040-1048.2006
Popis: Until recently, methicillin-resistant Staphylococcus aureus (MRSA) was considered the prototype of a hospital-acquired bacterial pathogen. However, recent reports have shown that MRSA has now emerged in the community. Characterization of specific markers for distinguishing the origin of isolates could contribute to improved knowledge of MRSA epidemiology. The release of whole-genome sequences of hospital- and community-acquired S. aureus strains allowed the development of whole-genome content analysis techniques, including microarrays. We developed a microarray composed of 8,191 open reading frame-specific oligonucleotides covering >99% of the four sequenced S. aureus genomes (N315, Mu50, MW2, and COL) to evaluate gene contents of hospital- and community-onset S. aureus strains. In parallel, pulsed-field gel electrophoresis, variable number of tandem repeats, antibiogram, staphylococcal cassette chromosome- mec element typing, and presence of the Panton-Valentine leukocidin gene were evaluated in a collection of 15 clinical isolates. Clusters obtained with microarrays showed a high degree of similarity with those obtained by pulsed-field gel electrophoresis or variable number of tandem repeats. Clusters clearly segregated hospital-onset strains from community-onset strains. Moreover, the microarray approach allowed definition of novel marker genes and chromosomal regions specific for given groups of isolates, thus providing better discrimination and additional information compared to pulsed-field gel electrophoresis and variable number of tandem repeats. Finally, the comparative genome hybridization approach unraveled the occurrence of multiple horizontal transfer events leading to community-onset MRSA as well as the need for a specific genetic background in recipient strains for both the acquisition and the stability of the mec element.
Databáze: OpenAIRE
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