Mature CD8(+) T lymphocyte response to viral infection during fetal life
| Autor: | Marchant, A., Appay, V., Sande, M., Dulphy, N., Liesnard, C., Kidd, M., Kaye, S., Ojuola, O., Gillespie, G. M. A., Vargas Cuero, A. L., Vincenzo Cerundolo, Callan, M., Mcadam, K. P. W. J., Rowland-Jones, S. L., Donner, C., Mcmichael, A. J., Whittle, H. |
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| Jazyk: | angličtina |
| Rok vydání: | 2003 |
| Předmět: |
Pore Forming Cytotoxic Proteins
Time Factors CD8 Antigens viruses chemical and pharmacologic phenomena CD8-Positive T-Lymphocytes Polymerase Chain Reaction Models Biological Article Immunophenotyping Fetus CD28 Antigens Pregnancy Histocompatibility Antigens Humans Vaccines Membrane Glycoproteins Perforin Infant Newborn virus diseases Cell Differentiation hemic and immune systems General Medicine Flow Cytometry Tumor Necrosis Factor Receptor Superfamily Member 7 Phenotype Cytomegalovirus Infections Commentary Leukocyte Common Antigens Female Immunization Peptides Immunologic Memory |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 1558-8238 0021-9738 |
| Popis: | Immunization of newborns against viral infections may be hampered by ineffective CD8(+) T cell responses. To characterize the function of CD8(+) T lymphocytes in early life, we studied newborns with congenital human cytomegalovirus (HCMV) infection. We demonstrate that HCMV infection in utero leads to the expansion and the differentiation of mature HCMV-specific CD8(+) T cells, which have similar characteristics to those detected in adults. High frequencies of HCMV-specific CD8(+) T cells were detected by ex vivo tetramer staining as early as after 28 weeks of gestation. During the acute phase of infection, these cells had an early differentiation phenotype (CD28(-)CD27(+)CD45RO(+), perforin(low)), and they acquired a late differentiation phenotype (CD28(-)CD27(-)CD45RA(+), perforin(high)) during the course of the infection. The differentiated cells showed potent perforin-dependent cytolytic activity and produced antiviral cytokines. The finding of a mature and functional CD8(+) T cell response to HCMV suggests that the machinery required to prime such responses is in place during fetal life and could be used to immunize newborns against viral pathogens. |
| Databáze: | OpenAIRE |
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