PrismPlus: a mouse line expressing distinct fluorophores in four different brain cell types
| Autor: | Heui Chang Lee, Ray Ward, Janak Gaire, Seth Currlin, Andrew J. Woolley, Justin C. Williams, Jason E. Coleman, Kevin J. Otto |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Genetically modified mouse Male Cell type Science Transgene Recombinant Fusion Proteins Central nervous system Green Fluorescent Proteins CX3C Chemokine Receptor 1 Gene Expression Mice Transgenic Biology Article 03 medical and health sciences Mice 0302 clinical medicine Bacterial Proteins Gene expression Brain Injuries Traumatic medicine Animals Transgenes Crosses Genetic Neurons Multidisciplinary Microglia Brain Founder Effect Electrodes Implanted Repressor Proteins Disease Models Animal Luminescent Proteins Oligodendroglia 030104 developmental biology medicine.anatomical_structure Astrocytes Medicine Immunohistochemistry Female Neuroscience 030217 neurology & neurosurgery Fluorescent tag |
| Zdroj: | Scientific Reports Scientific Reports, Vol 8, Iss 1, Pp 1-11 (2018) |
| ISSN: | 2045-2322 |
| Popis: | To screen the complex central nervous system (CNS) injury responses, we created a quadruple-labelled ‘PrismPlus’ mouse line with a genetically encoded distinct fluorescent tag in oligodendrocytes, microglia, neurons, and astrocytes. Cx3cr1-gfp and Prism mice originally developed by Jung et al., 2000 and Dougherty et al., 2012, respectively, were cross-bred. First, we confirmed the presence of fluorophores in appropriate cell types in PrismPlus mice. PrismPlus mice were then used to examine the cellular responses to brain implanted micro-devices. We observed an increase in microglial response at earlier time points as compared to 4 weeks, a progressive astrocytic response, and fewer neurons at the vicinity of an implanted device. These results are similar to what has been described in literature using other rodent strains, previously attainable only through time-consuming and variable immunohistochemistry methods. Finally, we demonstrate the compatibility of PrismPlus brain tissue with CLARITY, an advanced tissue clearing technique, opening the door to future thick tissue imaging studies. This report confirms PrismPlus transgenic fluorescence and highlights the utility of these mice to study CNS injuries. The work herein seeks to establish a novel transgenic mouse line to improve experimental scope, consistency, and efficiency for CNS researchers. |
| Databáze: | OpenAIRE |
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