A monoclonal antibody to DIII E protein allowing the differentiation of West Nile virus from other flaviviruses by a lateral flow assay
| Autor: | Ana Camuñas, María Paz Sánchez-Seco, Elisa Pérez-Ramírez, Belén Rebollo, Miguel Ángel Jiménez-Clavero, Teresa Pérez, Ángel Venteo, Francisco Llorente |
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| Rok vydání: | 2018 |
| Předmět: |
Monoclonal antibody
0301 basic medicine medicine.drug_class animal diseases viruses Cross Reactions Antibodies Viral Chromatography Affinity Epitope Epitopes 03 medical and health sciences Flaviviridae 0302 clinical medicine Viral Envelope Proteins Virology Chlorocebus aethiops medicine Animals Humans 030212 general & internal medicine Vero Cells chemistry.chemical_classification biology Flavivirus Antibodies Monoclonal virus diseases RNA virus Viral Load biochemical phenomena metabolism and nutrition biology.organism_classification nervous system diseases 030104 developmental biology chemistry Vero cell Immunochromatography Glycoprotein West Nile virus Viral load West Nile Fever |
| Zdroj: | Repositorio de Resultados de Investigación del INIA INIA: Repositorio de Resultados de Investigación del INIA Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria INIA |
| Popis: | West Nile Virus (WNV) belongs to the Flaviviridae family, genus Flavivirus, which includes other emerging arthropod-borne viruses (arboviruses) pathogenic for animals and/or humans. West Nile Virus is a genetically diverse RNA virus with at least 7 different recognized lineages. Following its recent introduction and subsequent expansion to the Americas, WNV is currently one of the most widely spread arboviruses in the world having recently re-emerged in the Mediterranean basin, Central and Eastern Europe. Laboratory tests are essential to confirm WNV infection and monoclonal antibodies represent useful tools for the development of diagnostic assays. A monoclonal antibody, 1D11, recognizing an epitope in the domain III of the envelope glycoprotein of WNV, was selected for this study. Its suitability to detect a range of WNV variants representative of its whole genetic range, and to differentiate it from other flaviviruses and arboviruses, was assessed by means of an immunochromatographic assay in an LFA format. A panel of cell culture supernatants infected with 9 different WNV isolates representing a wide range of genetic lineages, and 16 non-WNV arboviruses, including flaviviruses closely related to WNV, were tested. The mAb correctly detected all WNV strains, and did not react with any of the non-WNV arboviruses. |
| Databáze: | OpenAIRE |
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