Protein–polyelectrolyte complexes to improve the biological activity of proteins in layer-by-layer assemblies
| Autor: | A. vander Straeten, C. D'Haese, Anna Bratek-Skicki, Pierre Eloy, Christine C. Dupont-Gillain, Loïc Germain, Charles-André Fustin |
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| Rok vydání: | 2017 |
| Předmět: |
Materials science
Protein molecules Protein immobilization Layer by layer Biological activity 02 engineering and technology 010402 general chemistry 021001 nanoscience & nanotechnology Polyelectrolytes 01 natural sciences Polyelectrolyte 0104 chemical sciences Styrene chemistry.chemical_compound Immobilized Proteins Sulfonate chemistry Chemical engineering Polystyrenes Organic chemistry Muramidase General Materials Science Specific activity 0210 nano-technology |
| Zdroj: | Nanoscale |
| ISSN: | 2040-3372 2040-3364 |
| Popis: | A standard method of protein immobilization is proposed, based on the use of protein-polyelectrolyte complexes (PPCs) as building blocks for layer-by-layer assembly. Thicker multilayers, with a higher polyelectrolyte fraction, are obtained with PPCs compared to single protein molecules. Biological activity is not only maintained, but specific activity is also higher, as demonstrated for lysozyme-poly(styrene sulfonate) complexes. This is attributed to the more hydrated state of the assemblies. This new method of protein immobilization opens up perspectives for biotechnology and biomedical applications. |
| Databáze: | OpenAIRE |
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