Supplementation of Freezing Media with Cyclic Adenosine Monophosphate Analog and Isobutylmethylxanthine on Sperm Quality

Autor:	Hamid Piroozmanesh, Leila Naserpoor, Rahil Jannatifar
Rok vydání:	2020
Předmět:	camp analog (ibmx) Medicine (General) medicine.medical_specialty sperm quality General Medicine cryopreservation chemistry.chemical_compound R5-920 Endocrinology chemistry Internal medicine medicine Cyclic adenosine monophosphate Sperm quality asthenozoospermic
Zdroj:	Research in Molecular Medicine, Vol 8, Iss 4, Pp 201-208 (2020)
ISSN:	2322-1348 2322-133X
DOI:	10.32598/rmm.8.4.6
Popis:	Background: This study aimed to explore whether the addition of a cyclic adenosine monophosphate (cAMP) analog and isobutylmethylxanthine (IBMX) in freezing media improved sperm quality and what role cAMP has in this recovery. Materials and methods: ach semen sample was cryopreserved into four groups: fresh semen sample, as a control group, freezing medium + 2.5 mM cAMP analog and 0.2 mM IBMX, freezing medium + 12.5 mM cAMP analog and 0.2 mM IBMX, and freezing medium + 25 mM cAMP analog and 0.2 mM IBMX. Sperm parameters after post-thaw were analyzed according to WHO instruction (2010). Viability, acrosome reaction, and DNA damage levels of the samples were evaluated. Results: Our results indicated that the effective concentrations of 12.5 and 25 mM cAMP analog and 0.2 mM IBMX significantly improved the total motility, progressive motility, and viability of the frozen-thawed (P
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3b3df7f48f817f6e4fa322d3dab77227 https://doi.org/10.32598/rmm.8.4.6 Zobrazit plný text záznamu