Ultrasensitive detection of coliforms by means of direct asymmetric PCR combined with disposable magnetic amperometric genosensors
| Autor: | Pedro García, María Pedrero, José M. Pingarrón, Óscar A. Loaiza, Susana Campuzano |
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| Rok vydání: | 2009 |
| Předmět: |
DNA
Bacterial Colony Count Microbial Analytical chemistry Biosensing Techniques Polymerase Chain Reaction Sensitivity and Specificity Biochemistry Analytical Chemistry Magnetics chemistry.chemical_compound Escherichia coli Electrochemistry Environmental Chemistry Electrodes Spectroscopy Colony-forming unit Chromatography DNA–DNA hybridization Electrochemical detector Asymmetric PCR Amperometry Chemical sensor chemistry Gold Water Microbiology DNA |
| Zdroj: | The Analyst. 134:34-37 |
| ISSN: | 1364-5528 0003-2654 |
| Popis: | An extremely sensitive procedure for the isolation and detection of DNA from bacterial cell cultures is described. Direct asymmetric PCR amplified products from E. coli cultures are specifically detected, at a concentration level as low as 0.01 cfu mL(-1) (cfu, colony forming unit), using disposable magnetic DNA hybridization amperometric sensors with no need for culture preconcentration steps. |
| Databáze: | OpenAIRE |
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