Imaging proteins at the single-molecule level
| Autor: | Jean-Nicolas Longchamp, Conrad Escher, Stephan Rauschenbach, Sabine Abb, Klaus Kern, Tatiana Latychevskaia, Hans-Werner Fink |
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| Přispěvatelé: | University of Zurich, Longchamp, Jean-Nicolas |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Electrospray 530 Physics low-energy electron holography Holography Nanotechnology 10192 Physics Institute 02 engineering and technology Molecular nanotechnology Electron holography 03 medical and health sciences Ion beam deposition Molecule structural biology single protein imaging preparative mass spectrometry Molecular Biology 1000 Multidisciplinary Multidisciplinary biology Chemistry Cytochrome c Resolution (electron density) 021001 nanoscience & nanotechnology 030104 developmental biology Structural biology Physical Sciences Biophysics biology.protein microscopy 0210 nano-technology |
| Popis: | Imaging single proteins has been a long-standing ambition for advancing various fields in natural science, as for instance structural biology, biophysics, and molecular nanotechnology. In particular, revealing the distinct conformations of an individual protein is of utmost importance. Here, we show the imaging of individual proteins and protein complexes by low-energy electron holography. Samples of individual proteins and protein complexes on ultraclean freestanding graphene were prepared by soft-landing electrospray ion beam deposition, which allows chemical- and conformational-specific selection and gentle deposition. Low-energy electrons do not induce radiation damage, which enables acquiring subnanometer resolution images of individual proteins (cytochrome C and BSA) as well as of protein complexes (hemoglobin), which are not the result of an averaging process. |
| Databáze: | OpenAIRE |
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