H2-M3–Restricted T Cells in Bacterial Infection
| Autor: | Kristen M. Kerksiek, Dirk H. Busch, Eric G. Pamer, Ingrid M. Pilip, S E Allen |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1999 |
| Předmět: |
Protein Conformation
T cell Immunology CD1 CD8-Positive T-Lymphocytes In Vitro Techniques Major histocompatibility complex cytotoxic T lymphocytes Lymphocyte Activation 03 medical and health sciences Epitopes Mice 0302 clinical medicine MHC class I medicine Immunology and Allergy Cytotoxic T cell Animals Listeriosis H2-M3 030304 developmental biology DNA Primers 0303 health sciences Antigens Bacterial Mice Inbred BALB C Mice Inbred C3H major histocompatibility complex class Ib molecules biology Base Sequence bacterial infection Histocompatibility Antigens Class II MHC restriction Listeria monocytogenes 3. Good health Mice Inbred C57BL medicine.anatomical_structure biology.protein Original Article Female Memory T cell Immunologic Memory CD8 030215 immunology |
| Zdroj: | The Journal of Experimental Medicine |
| ISSN: | 1540-9538 0022-1007 |
| Popis: | Major histocompatibility complex (MHC) class Ib molecules have been implicated in CD8+ T cell–mediated defenses against intracellular bacterial infection, but the relative importance of MHC class Ib–restricted T cells in antimicrobial immunity is unknown. In this report, we use MHC tetramers to characterize T cell responses restricted by H2-M3, an MHC class Ib molecule that selectively presents N-formyl peptides. We find that sizeable H2-M3–restricted T cell responses, occurring earlier than MHC class Ia–restricted T cell responses, are mounted after primary infection with the intracellular bacterium Listeria monocytogenes. These H2-M3–restricted T cells are cytolytic and produce interferon γ. However, after a second L. monocytogenes infection, H2-M3–restricted memory T cell responses are minor in comparison to the much larger MHC class Ia–restricted responses. This first direct characterization of an MHC class Ib–restricted T cell response indicates that CD8+ T cells responding to L. monocytogenes infection can be divided into two groups: H2-M3–restricted responses, which provide rapid and quantitatively substantial effector function during primary infections but contribute relatively little to memory responses, and MHC class Ia–restricted responses, which expand later during primary infection but form memory T cells that respond rapidly and dramatically in response to subsequent infections by the same pathogen. |
| Databáze: | OpenAIRE |
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