Enzymatic Synthesis of Mannosyl-cyclodextrin by α-Mannosidase from Jack Bean
| Autor: | Koki Fujita, Toshiko Tanimoto, Hirofumi Nakano, Kyoko Koizumi, Sumio Kitahata, Koji Hara, Yukio Kondo, Kenichi Hamayasu, Hitoshi Hashimoto |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
chemistry.chemical_classification
Mannosidase Glycosylation biology Cyclodextrin Chemistry Stereochemistry Organic Chemistry Mannose General Medicine Oligosaccharide biology.organism_classification Applied Microbiology and Biotechnology Biochemistry Analytical Chemistry chemistry.chemical_compound Enzyme Canavalia ensiformis Yield (chemistry) Molecular Biology Biotechnology Nuclear chemistry |
| Zdroj: | Bioscience, biotechnology, and biochemistry. 61(5) |
| ISSN: | 1347-6947 |
| Popis: | Mannosylated derivatives of cyclodextrins (CDs), mannosyl-α, β, and γCD were synthesized from a mixture of mannose and α, β, and γCD by the reverse action of α-mannosidase from jack bean, respectively. Their structures were analyzed by FAB-MS and 13C-NMR spectroscopies, and they were identified as 6-O-α-d-mannosyl-α, β, and γCD. The optimum conditions for the production of 6-O-α-d-mannosyl-αCD by α-mannosidase were examined. Optimum pH and temperature were pH 4.5 and 60°C, respectively. Yield of mannosyl-αCD increased with increasing mannose concentration and reached more than 35% (mol/mol) at the concentration of 2 m mannose and 0.4 m αCD. |
| Databáze: | OpenAIRE |
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