Protection against lethal Sendai virus infection by in vivo priming of virus-specific cytotoxic T lymphocytes with a free synthetic peptide
| Autor: | W. M. Kast, D. Kolakofsky, R. H. Meloen, J. Curren, Cornelis J. M. Melief, H. J. J. Blom, L. Roux, A. C. Voordouw |
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| Rok vydání: | 1991 |
| Předmět: |
Cytotoxicity
Immunologic Virus-specific Cytotoxic T-lymphocytes Paramyxoviridae viruses Mice Inbred Strains Vaccinia virus Recombinant virus Virus Microbiology Mice Animals Poxviridae Orthopoxvirus Cells Cultured Vaccines Synthetic Paramyxoviridae Infections Multidisciplinary biology Viral Vaccines biology.organism_classification Virology Sendai virus Clone Cells Parainfluenza Virus 1 Human CTL T-Lymphocytes Cytotoxic Research Article |
| Zdroj: | Proceedings of the National Academy of Sciences. 88:2283-2287 |
| ISSN: | 1091-6490 0027-8424 |
| Popis: | The only peptide of Sendai virus that is recognized by cytotoxic T lymphocytes (CTL) in B6 mice was found with (i) the use of recombinant vaccinia virus constructs containing separate genes of Sendai virus and (ii) a set of overlapping peptides completely spanning the identified nucleoprotein (NP) gene product. This immunodominant NP peptide is recognized by Sendai virus-specific CTL that are known to have therapeutic effects in vivo. By subcutaneous immunization, this peptide induced Sendai virus and NP peptide-specific CTL memory responses in vivo. Most importantly, mice that had been immunized with this peptide were protected against a lethal virus dose, indicating that viral peptides can be used as antiviral T-cell vaccines. The induction of T-cell memory by free peptide immunization potentially has wide applicability in biology and medicine, including protection against infectious disease. |
| Databáze: | OpenAIRE |
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