Sex differences in microRNA expression in first and third trimester human placenta
| Autor: | Amy E Flowers, Tania L Gonzalez, Nikhil V Joshi, Laura E Eisman, Ekaterina L Clark, Rae A Buttle, Erica Sauro, Rosemarie DiPentino, Yayu Lin, Di Wu, Yizhou Wang, Chintda Santiskulvong, Jie Tang, Bora Lee, Tianyanxin Sun, Jessica L Chan, Erica T Wang, Caroline Jefferies, Kate Lawrenson, Yazhen Zhu, Yalda Afshar, Hsian-Rong Tseng, John Williams, Margareta D Pisarska |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Male
human transcriptome developmental epigenetics Placenta Pregnancy Trimester Third chorionic villous sampling Reproductive health and childbirth Medical and Health Sciences placenta sex differences stable miRNAs Epigenesis Genetic Genetic Pregnancy Genetics Humans Obstetrics & Reproductive Medicine First Third Pediatric screening and diagnosis Sex Characteristics microRNA Human Genome miRNome sexually dimorphic normative miRNA atlas Cell Biology General Medicine Perinatal Period - Conditions Originating in Perinatal Period Biological Sciences 4.1 Discovery and preclinical testing of markers and technologies Detection MicroRNAs Pregnancy Trimester First Good Health and Well Being Reproductive Medicine Female Pregnancy Trimester Epigenesis Biotechnology Research Article |
| Zdroj: | Biol Reprod Biology of reproduction, vol 106, iss 3 |
| Popis: | Maternal and fetal pregnancy outcomes related to placental function vary based on fetal sex, which may be due to sexually dimorphic epigenetic regulation of RNA expression. We identified sexually dimorphic miRNA expression throughout gestation in human placentae. Next-generation sequencing identified miRNA expression profiles in first and third trimester uncomplicated pregnancies using tissue obtained at chorionic villous sampling (n = 113) and parturition (n = 47). Sequencing analysis identified 986 expressed mature miRNAs from female and male placentae at first and third trimester (baseMean>10). Of these, 11 sexually dimorphic (FDR 10, with 508 DE miRNAs in common between female-specific and male-specific analysis (269 upregulated in first trimester, 239 upregulated in third trimester). Of those, miR-4483 had the highest fold changes across gestation. There were 62.5% more female exclusive differences with fold change>2 across gestation than male exclusive (52 miRNAs vs 32 miRNAs), indicating miRNA expression across human gestation is sexually dimorphic. Pathway enrichment analysis identified significant pathways that were differentially regulated in first and third trimester as well as across gestation. This work provides the normative sex dimorphic miRNA atlas in first and third trimester, as well as the sex-independent and sex-specific placenta miRNA atlas across gestation, which may be used to identify biomarkers of placental function and direct functional studies investigating placental sex differences. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|