Gradient in cytoplasmic pressure in germline cells controls overlying epithelial cell morphogenesis

Autor: Pradeep Das, Arezki Boudaoud, Muriel Grammont, Laurie-Anne Lamire, Stève de Bossoreille, Blandine Vergier, Annamaria Kiss, Pascale Milani, Gaël Runel, David Cluet, Leticia Arias
Přispěvatelé: Reproduction et développement des plantes (RDP), École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), École normale supérieure - Lyon (ENS Lyon), Laboratoire de biologie et modélisation de la cellule (LBMC UMR 5239), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), French National Research Agency (ANR)European CommissionBlanc 12-SVSE-0023-01Centre National pour la Recherche Scientifique Ecole Normale Superieure of Lyon, Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-École normale supérieure - Lyon (ENS Lyon)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), École normale supérieure de Lyon (ENS de Lyon)
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0301 basic medicine
Cytoplasm
Epithelial cell morphogenesis
[SDV]Life Sciences [q-bio]
Microscopy
Atomic Force
Biochemistry
Infographics
Nurse cell
Germline
Epithelium
Stiffness
0302 clinical medicine
Oogenesis
Ovarian Follicle
Transforming Growth Factor beta
Animal Cells
Medicine and Health Sciences
Drosophila Proteins
Biology (General)
Data Management
Microscopy
General Neuroscience
Physics
Cell Polarity
Classical Mechanics
Cell Differentiation
Cell biology
Biomechanical Phenomena
Atomic Force Microscopy
medicine.anatomical_structure
Drosophila melanogaster
OVA
Physical Sciences
Female
Cellular Types
Anatomy
General Agricultural and Biological Sciences
Graphs
Signal Transduction
Research Article
Computer and Information Sciences
QH301-705.5
Materials Science
Material Properties
Morphogenesis
Biology
Research and Analysis Methods
Models
Biological
Green Fluorescent Protein
General Biochemistry
Genetics and Molecular Biology
03 medical and health sciences
medicine
Pressure
Animals
Mechanical Properties
Ovarian follicle
Cell Shape
General Immunology and Microbiology
Scanning Probe Microscopy
Data Visualization
Biology and Life Sciences
Proteins
Epithelial Cells
Cell Biology
Oocyte
Luminescent Proteins
030104 developmental biology
Germ Cells
Biological Tissue
Oocytes
Mechanical Tension
030217 neurology & neurosurgery
Zdroj: PLoS Biology, Vol 18, Iss 11, p e3000940 (2020)
PLoS Biology
PLoS Biology, Public Library of Science, 2020, 18 (11), pp.e3000940. ⟨10.1371/journal.pbio.3000940⟩
PLoS Biology, 2020, 18 (11), pp.e3000940. ⟨10.1371/journal.pbio.3000940⟩
ISSN: 1545-7885
1544-9173
Popis: It is unknown how growth in one tissue impacts morphogenesis in a neighboring tissue. To address this, we used the Drosophila ovarian follicle, in which a cluster of 15 nurse cells and a posteriorly located oocyte are surrounded by a layer of epithelial cells. It is known that as the nurse cells grow, the overlying epithelial cells flatten in a wave that begins in the anterior. Here, we demonstrate that an anterior to posterior gradient of decreasing cytoplasmic pressure is present across the nurse cells and that this gradient acts through TGFβ to control both the triggering and the progression of the wave of epithelial cell flattening. Our data indicate that intrinsic nurse cell growth is important to control proper nurse cell pressure. Finally, we reveal that nurse cell pressure and subsequent TGFβ activity in the stretched cells combine to increase follicle elongation in the anterior, which is crucial for allowing nurse cell growth and pressure control. More generally, our results reveal that during development, inner cytoplasmic pressure in individual cells has an important role in shaping their neighbors.
A major feature of ovarian follicular development in Drosophila is the progressive flattening of anterior epithelial cells that surround the germline. A combination of mechanical measurements, live imaging, and genetic approaches reveals that germline cytoplasmic pressure is graded and controls this flattening; this germline pressure gradient, in concert with basement membrane stiffness, then guides follicle elongation.
Databáze: OpenAIRE
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