Immunological memory and neutralizing activity to a single dose of COVID-19 vaccine in previously infected individuals
| Autor: | Gujjarlapudi Deepika, Duvvur Nageshwar Reddy, Vemula Venkata Krishna, Kottapalli Pragathi, Yelamanchili Sadhana, V. V. Ravikanth, Mitnala Sasikala, Jaggaiahgari Shashidhar |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Microbiology (medical) COVID-19 Vaccines Coronavirus disease 2019 (COVID-19) Short Communication 030106 microbiology C-C chemokine receptor type 7 Infectious and parasitic diseases RC109-216 CD8-Positive T-Lymphocytes Antibodies Viral Flow cytometry Memory B-cells 03 medical and health sciences 0302 clinical medicine Memory T-cells Medicine Humans 030212 general & internal medicine Neutralizing antibody CD20 biology medicine.diagnostic_test business.industry SARS-CoV-2 COVID-19 General Medicine Vaccination Infectious Diseases Immunology biology.protein Antibody business Immunologic Memory CD8 |
| Zdroj: | International Journal of Infectious Diseases, Vol 108, Iss, Pp 183-186 (2021) International Journal of Infectious Diseases |
| ISSN: | 1201-9712 |
| Popis: | Background The efficacy of COVID-19 vaccines to generate immunological memory post-vaccination has not previously been studied. Objective To assess immunological memory in previously SARS-CoV-2 infected individuals after a single dose of mRNA vaccine. Patients and methods Healthcare workers (n = 280) were enrolled after obtaining written informed consent and grouped under previously infected and no prior exposure (reverse transcription-polymerase chain reaction positive and negative, respectively). Blood was drawn at baseline and post-vaccination (single dose of COVISHIELD) for enumerating neutralizing antibodies by chemiluminescence and memory T- and B-cells by flow cytometry. Results Post vaccination, compared with the no prior exposure group, the previously infected group had higher levels of: antibody response (1124.73 ± 869.13 vs 94.23 ± 140.06 AU/ml, p = 0.0001); CD4 memory T-cells: central memory CCR7+CD45RA- (p = 0.0001), effector memory CCR7-/CD45RA- (p = 0.01); total CD8+ T-cells (p = 0.004); CD8+ naive T-cells CCR7+CD45RA+ (p = 0.01); and memory B-cells CD20+CD27+ (p = 0.0001). Discussion Single-dose vaccination elicited higher neutralizing antibody response and protective immunity in individuals who had recovered from SARS-CoV-2 infection compared with those with no prior exposure. |
| Databáze: | OpenAIRE |
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