The antioxidative properties of S-allyl cysteine not only influence somatic cells but also improve early embryo cleavage in pigs
| Autor: | David Němeček, Ivona Heroutová, Veronika Kučerová Chrpová, Jaroslav Petr, Kateřina Adámková, Jan Nevoral, Markéta Sedmíková, M. Dvořáková, Tereza Krejcova |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Veterinary Medicine Oocyte Antioxidant Somatic cell medicine.medical_treatment S-Allyl cysteine lcsh:Medicine Biology Bioinformatics General Biochemistry Genetics and Molecular Biology Andrology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine medicine Garlic chemistry.chemical_classification Reactive oxygen species General Neuroscience lcsh:R food and beverages Embryo General Medicine S-allyl cysteine In vitro 030104 developmental biology medicine.anatomical_structure chemistry 030220 oncology & carcinogenesis Pigs General Agricultural and Biological Sciences Cysteine Biotechnology Developmental Biology |
| Zdroj: | PeerJ, Vol 4, p e2280 (2016) PeerJ |
| ISSN: | 2167-8359 |
| Popis: | In vitro cultivation systems for oocytes and embryos are characterised by increased levels of reactive oxygen species (ROS), which can be balanced by the addition of suitable antioxidants. S-allyl cysteine (SAC) is a sulfur compound naturally occurring in garlic (Allium sativum), which is responsible for its high antioxidant properties. In this study, we demonstrated the capacity of SAC (0.1, 0.5 and 1.0 mM) to reduce levels of ROS in maturing oocytes significantly after 24 (reduced by 90.33, 82.87 and 91.62%, respectively) and 48 h (reduced by 86.35, 94.42 and 99.05%, respectively) cultivation, without leading to a disturbance of the standard course of meiotic maturation. Oocytes matured in the presence of SAC furthermore maintained reduced levels of ROS even 22 h after parthenogenic activation (reduced by 66.33, 61.64 and 57.80%, respectively). In these oocytes we also demonstrated a growth of early embryo cleavage rate (increased by 33.34, 35.00 and 35.00%, respectively). SAC may be a valuable supplement to cultivation media. |
| Databáze: | OpenAIRE |
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