Interdependence of cortical thymic epithelial cell differentiation and T-lineage commitment
| Autor: | Carla Carter, Claudio J. Conti, David B. Klug, Ellen R. Richie, Dennis R. Roop, Elizabeth Crouch |
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| Rok vydání: | 1998 |
| Předmět: |
Lineage (genetic)
T cell TEC Cellular differentiation Receptors Antigen T-Cell alpha-beta T-Lymphocytes education Receptors Antigen T-Cell Mice Transgenic Thymus Gland Biology Recombination-activating gene Mice T-Lymphocyte Subsets TheoryofComputation_ANALYSISOFALGORITHMSANDPROBLEMCOMPLEXITY medicine Animals Humans Homeodomain Proteins Mice Knockout Multidisciplinary FOXN1 hemic and immune systems Cell Differentiation Epithelial Cells Receptors Antigen T-Cell gamma-delta Biological Sciences Immunohistochemistry Cell biology Transplantation Mice Inbred C57BL Thymocyte medicine.anatomical_structure Immunology Keratins tissues |
| Zdroj: | Proceedings of the National Academy of Sciences of the United States of America. 95(20) |
| ISSN: | 0027-8424 |
| Popis: | Thymocyte and thymic epithelial cell (TEC) development are interdependent processes. Although lineage relationships among progressively maturing thymocyte subsets have been characterized, the developmental relationships among TEC subsets are obscure. Because epithelial cells express distinct keratin (K) species as a function of differentiation stage and proliferative status, we used K expression patterns to identify mouse TEC subsets and determine their lineage relationships. As expected, cortical and medullary TEC subsets express distinct K expression patterns in the normal thymus. However, we detected two distinct cortical TEC subsets, a major K8+K5−subset and a minor K8+K5+subset, which is highly represented at the cortico-medullary junction. Both cortical TEC subsets are also present in recombination activating gene 1 (RAG-1−/−) and TCRβxδ−/−thymi in which T-cell development is blocked at the CD4−CD8−CD25+CD44−pre-T cell stage. In contrast, K8+K5+TECs predominate in the thymi of human CD3ɛ transgenic mice in which thymocyte development is blocked at an earlier CD4−CD8−CD25−CD44+stage. Transplantation of newborn human CD3ɛ transgenic thymi under the kidney capsule of RAG-1−/−mice results in the emergence of K8+K5−TECs concomitant with the appearance of CD25+thymocytes. Together, the data suggest that cortical TEC development proceeds from a K8+K5+precursor subset to a K8+K5−stage in a differentiation process concomitant with T-cell lineage commitment. |
| Databáze: | OpenAIRE |
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