Distribution and density of CD1a+ and CD83+ dendritic cells in HPV-associated laryngeal papillomas
| Autor: | Polina V Lukashenko, Valentin Gerein, Sergey I. Bakanov, Sergey Kovalenko, Alesia Romanovskaya, Herbert Pfister, Iouri L. Soldatski |
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| Rok vydání: | 2008 |
| Předmět: |
Male
Pathology medicine.medical_specialty Adolescent Immunoglobulins Cell Count Antigens CD1 Young Adult Antigens CD Immunopathology Biopsy medicine Humans Tissue Distribution Child Laryngeal Neoplasms Retrospective Studies Lamina propria Membrane Glycoproteins medicine.diagnostic_test Papilloma Benign Laryngeal Neoplasm business.industry Human papillomavirus 11 Papillomavirus Infections Infant General Medicine Dendritic Cells medicine.disease Human papillomavirus 6 Epithelium medicine.anatomical_structure Otorhinolaryngology Child Preschool Pediatrics Perinatology and Child Health Female Recurrent Respiratory Papillomatosis Larynx business Laryngeal papillomatosis |
| Zdroj: | International journal of pediatric otorhinolaryngology. 73(2) |
| ISSN: | 0165-5876 |
| Popis: | Summary Background Respiratory papillomatosis associated with human papilloma virus (HPV) infection is the most common benign laryngeal neoplasm. The age of patients at disease onset, HPV type, number of surgeries are well known prognostic factors of the disease course. The correlation between dendritic cell (DC) density in tumor tissue and clinical prognosis was established. Aim The aim of our study was to estimate the density of DC in laryngeal papillomas associated with HPV types 6/11 infection and to evaluate the relationship between the number of DC and the disease severity. Materials and methods Our study included 40 randomly selected biopsy specimens from patients with HPV-positive laryngeal papillomatosis aged from 1.7 to 20 year. DC were immunohistochemically labelled with anti-CD1a antibodies and anti-CD83 antibodies. The density of DC was analysed in epithelial layer and lamina propria. Results In the epithelial layer of papillomas the number of CD1a+ and CD83+ DC was 86.2 (47.5–119.9) cells/mm2 and 2.6 (0.6–7.9) cells/mm2, respectively. In lamina propria – 15.3 (5.1–27.9) and 16.0 (6.7–33.2) cells/mm2. For subgroups of patients with high number of operations (more than 3), early disease onset (children under 3 years of age) and lingering duration of disease (more than 1 year) we detected an increase of CD83+ DC in the epithelial layer. However, our data did not demonstrate a statistically significant difference in CD1a+ DC count neither in the epithelium nor in the lamina propria. Probably, the increase of CD83+ DC density in epithelial layer of patients with severe course of disease can be an evidence of impaired migration of matured DC. |
| Databáze: | OpenAIRE |
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