In Situ Localization and Quantification of mRNA for 92-kD Type IV Collagenase and Its Inhibitor in Aneurysmal, Occlusive, and Normal Aorta
| Autor: | Maria Cipollone, William D. McMillan, Bruce K. Patterson, Richard R. Keen, William H. Pearce, Vera P. Shively |
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| Rok vydání: | 1995 |
| Předmět: |
Pathology
medicine.medical_specialty Vascular smooth muscle Gene Expression In situ hybridization In Vitro Techniques Biology Muscle Smooth Vascular Aortic aneurysm medicine.artery Gene expression medicine Humans Collagenases RNA Messenger Aorta Cells Cultured In Situ Hybridization Glycoproteins Messenger RNA Tissue Inhibitor of Metalloproteinases Tissue inhibitor of metalloproteinase medicine.disease Aneurysm Molecular biology Matrix Metalloproteinase 9 cardiovascular system Interstitial collagenase Cardiology and Cardiovascular Medicine |
| Zdroj: | Arteriosclerosis, Thrombosis, and Vascular Biology. 15:1139-1144 |
| ISSN: | 1524-4636 1079-5642 |
| DOI: | 10.1161/01.atv.15.8.1139 |
| Popis: | Abstract Ninety-two-kilodalton type IV collagenase (MMP-9) is present in aortic aneurysms and may be important to the pathogenesis of this disease. Alteration in expression of MMP-9 or its inhibitor, the tissue inhibitor of metalloproteinase type 1 (TIMP-1), could increase degradation of extracellular matrix and lead to aneurysm formation. The purpose of this study was (1) to measure tissue levels of MMP-9 and TIMP-1 mRNA in aneurysmal (AAA), atherosclerotic occlusive (AOD), and normal (NL) human infrarenal aorta; (2) to test for their expression by cultured AAA and NL vascular smooth muscle cells (VSMCs); and (3) to locate in situ the cells responsible for mRNA production within AAA, AOD, and NL aortic wall. Total RNA extracted from AAA (n=8), AOD (n=8), and NL (n=7) tissue was subjected to Northern analysis. Signals for MMP-9 and TIMP-1 were normalized to α-tubulin. Mean values±SEM were compared by ANOVA. NL and AAA VSMCs were cultured, passaged, and grown to confluence before RNA extraction and Northern analysis. In situ hybridization with digoxigenin-labeled RNA probes localized cells responsible for MMP-9 and TIMP-1 mRNA expression within sections of AAA (n=5), AOD (n=2), and NL (n=2) aorta. MMP-9 mRNA levels were significantly greater in AAA (0.855±0.180) than NL (0.046±0.23) ( P |
| Databáze: | OpenAIRE |
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