Sensitive analysis and pharmacokinetic study of the isomers paeoniflorin and albiflorin after oral administration of Total Glucosides Of White Paeony Capsule in rats
| Autor: | Yuqing Zhao, Pei Wang, Hongbo Wang, Runbin Sun, Jingqiu Huang, Ying Peng, Guangji Wang, Na Yang, Fei Fei, Shu-Yao Wang, Siqi Feng, Xiaoyi Yu, Hailing Yang, Jiye Aa |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Bridged-Ring Compounds Male Clinical Biochemistry Cmax Administration Oral Pharmacology Tandem mass spectrometry Paeonia 01 natural sciences Biochemistry Analytical Chemistry Excretion 03 medical and health sciences chemistry.chemical_compound Pharmacokinetics Glucosides Isomerism Liquid chromatography–mass spectrometry Oral administration Limit of Detection Tandem Mass Spectrometry Animals Chromatography Chemistry 010401 analytical chemistry Capsule Reproducibility of Results Cell Biology General Medicine Paeoniflorin 0104 chemical sciences Rats 030104 developmental biology Linear Models Monoterpenes Female Chromatography Liquid Drugs Chinese Herbal |
| Zdroj: | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences. 1022 |
| ISSN: | 1873-376X |
| Popis: | A sensitive and reliable method using liquid chromatography tandem mass spectrometry (LC-MS/MS) was established for the simultaneous assay of paeoniflorin and albiflorin in bio-samples of rats after liquid-liquid extraction with ethylacetate. For the first time, the developed method was validated and successfully applied to the pharmacokinetics study of paeoniflorin and albiflorin after oral administration of Total Glucosides Of White Paeony Capsule (TGP). Relative to the intravenous injection, the absolute bio-availabilities of paeoniflorin and albiflorin were 2.8 and 1.7%, while their excretion in feces was 43.06 and 40.87%, respectively. Both paeoniflorin and albiflorin showed dose-dependent exposure in plasma, with a half-life of approximately 1.8h. No significant differences were observed between a single equal dose of paeoniflorin or albiflorin and that of TGP for the pharmacokinetic parameters, including AUC, T1/2 and Cmax. Paeoniflorin and albiflorin were exposed at high levels in immune relevant organ/tissues, such as the spleen, thymus and bone, which could facilitate immuno-regulatory activities. |
| Databáze: | OpenAIRE |
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