A self-renewing division of zebrafish Müller glial cells generates neuronal progenitors that require N-cadherin to regenerate retinal neurons
| Autor: | Linda K. Barthel, Mikiko Nagashima, Pamela A. Raymond |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Retinal Ganglion Cells
Heterozygote Interkinetic nuclear migration Neurogenesis Ependymoglial Cells Neuroepithelial Cells Biology Models Biological chemistry.chemical_compound Neural Stem Cells Cell Adhesion medicine Animals Regeneration Ouabain Molecular Biology Zebrafish Retinal regeneration Multipotent Stem Cells Asymmetric Cell Division Cell Cycle Retinal Cell Dedifferentiation Zebrafish Proteins Cadherins Stem Cells and Regeneration Neural stem cell Cell biology Neuroepithelial cell medicine.anatomical_structure nervous system chemistry Multipotent Stem Cell Muller glia Biomarkers Photoreceptor Cells Vertebrate Retinal Neurons Developmental Biology |
| Zdroj: | Development. 140:4510-4521 |
| ISSN: | 1477-9129 0950-1991 |
| DOI: | 10.1242/dev.090738 |
| Popis: | Müller glia function as retinal stem cells in adult zebrafish. In response to loss of retinal neurons, Müller glia partially dedifferentiate, re-express neuroepithelial markers and re-enter the cell cycle. We show that the immunoglobulin superfamily adhesion molecule Alcama is a novel marker of multipotent retinal stem cells, including injury-induced Müller glia, and that each Müller glial cell divides asymmetrically only once to produce an Alcama-negative, proliferating retinal progenitor. The initial mitotic division of Müller glia involves interkinetic nuclear migration, but mitosis of retinal progenitors occurs in situ. Rapidly dividing retinal progenitors form neurogenic clusters tightly associated with Alcama/N-cadherin-labeled Müller glial radial processes. Genetic suppression of N-cadherin function interferes with basal migration of retinal progenitors and subsequent regeneration of HuC/D+ inner retinal neurons. |
| Databáze: | OpenAIRE |
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