The stratum corneum transcriptome in atopic dermatitis can be assessed by tape stripping
| Autor: | Thomas Litman, Niels Morling, Julie Sølberg, Stine B Jacobsen, Jeppe Dyrberg Andersen, Marie-Louise Kampmann, Nina H. Ulrich, Jeanne D. Johansen, Malin G. Ahlström, Jacob P. Thyssen |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Adult Male Pathology medicine.medical_specialty Time Factors Biopsy Dermatology Biochemistry Dermatitis Atopic Specimen Handling Transcriptome 030207 dermatology & venereal diseases 03 medical and health sciences Young Adult 0302 clinical medicine Dermis medicine Stratum corneum Humans RNA-Seq Molecular Biology integumentary system medicine.diagnostic_test business.industry Shotgun sequencing Microbiota Atopic dermatitis Middle Aged medicine.disease Healthy Volunteers 030104 developmental biology medicine.anatomical_structure Hand eczema Case-Control Studies Female Epidermis business Biomarkers |
| Zdroj: | Journal of dermatological science. 101(1) |
| ISSN: | 1873-569X |
| Popis: | Background Skin biopsies represent a gold standard in skin immunology and pathology but can cause pain and induce scarring. Non-invasive techniques will facilitate study recruitment of e.g. patients with paediatric atopic dermatitis (AD), hand eczema or facial dermatitis. Objective By RNA sequencing, we examined whether the stratum corneum transcriptome in AD skin can be assessed by tape stripping, as compared to the epidermal transcriptome of AD in skin biopsies. To make the procedure clinically relevant tape strips were stored and shipped at room temperature for up to 3 days. Methods Nine adult Caucasian AD patients and three healthy volunteers were included. Tape samples were collected from non-lesional and lesional skin. Biopsies were collected from lesional skin and were split into epidermis and dermis. Total RNA was extracted, and shotgun sequencing was performed. Results Shotgun sequencing could be performed on skin cells obtained from two consecutive tape strips which had been stored and shipped at room temperature for up to three days. The most prominent differences between the tape strip and biopsy derived transcriptome were due to structural genes, while established molecular markers of AD, including CCL17, CCL22, IL17A and S100A7-S100A9, were also identified in tape strip samples. Furthermore, the tape strip derived transcriptome showed promise in also analysing the skin microbiome. Conclusion Our study shows that the stratum corneum (SC) transcriptome of AD can be assessed by tape stripping the skin, supporting that this method may be central in future skin biomarker research. NCBI GEO data accession: GSE160501 |
| Databáze: | OpenAIRE |
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