Infectivity of the Highly Transformable BBE02−lp56−Mutant ofBorrelia burgdorferi, the Lyme Disease Spirochete, via Ticks
| Autor: | Kathrine M Phillippi-Falkenstein, Mary B. Jacobs, Mario T. Philipp, Steven J. Norris |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Immunology
Spirochaetaceae Biology Tick Microbiology Mice Plasmid Lyme disease medicine Animals Borrelia burgdorferi Infectivity Lyme Disease Mice Inbred C3H Ixodes Bacterial Infections biology.organism_classification medicine.disease Virology Infectious Diseases Ixodes scapularis Mutation Female Parasitology Transformation Bacterial Plasmids |
| Zdroj: | Infection and Immunity. 74:3678-3681 |
| ISSN: | 1098-5522 0019-9567 |
| DOI: | 10.1128/iai.00043-06 |
| Popis: | InfectiousBorrelia burgdorferistrains that have increased transformability with the shuttle vector pBSV2 were recently constructed by inactivating the gene encoding BBE02, a putative restriction-modification gene product expressed by the linear plasmid lp25 (Kawabata et al., Infect. Immun. 72:7147-7154, 2004). The absence of the linear plasmid lp56, which carries another putative restriction-modification gene, further enhanced transformation rates. The infectivity of these mutants was assessed previously in mice that were inoculated with needle and syringe and was found to be equivalent to that of wild-type spirochetes. Here we examined the infectivity of spirochetes to ticks after capillary inoculation ofIxodes scapularisnymphs and the subsequent spirochetal infectivity to mice via ticks by usingB. burgdorferiB31 clonal isolates lacking lp56 and/or BBE02. The absence of lp56 (but not BBE02) correlated with a lower number of spirochetes in ticks after feeding on mice; this plasmid thus may play a role, albeit not an essential one, in supporting spirochetal survival in the feeding tick. Importantly, however, the absence of lp56 and BBE02 did not detectably influence infectivity to mice via ticks. |
| Databáze: | OpenAIRE |
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