High efficiency two-photon uncaging coupled by the correction of spontaneous hydrolysis
| Autor: | Csilla Lukácsné Haveland, Attila Kaszás, Gergely F. Turi, Gergely Katona, Balázs Rózsa, Zoltán Szadai, Ibolya Molnár-Perl, Dénes Pálfi, Milán Szőri, Béla Viskolcz, Attila Potor, Balázs Chiovini, Péter Ábrányi-Balogh, Imre G. Csizmadia, Orsolya Frigyesi, Zoltán Mucsi, Gergely Szalay, A. Vasanits-Zsigrai, Miklós Madarász |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Chemistry Organic Chemistry Intact brain Elimination method Biochemistry 03 medical and health sciences Hydrolysis 030104 developmental biology 0302 clinical medicine Neuronal communication Two-photon excitation microscopy Quantum chemical modelling Biophysics Molecule Physical and Theoretical Chemistry Spontaneous hydrolysis 030217 neurology & neurosurgery |
| Zdroj: | Organic & Biomolecular Chemistry |
| ISSN: | 1477-0539 |
| Popis: | Two-photon (TP) uncaging of neurotransmitter molecules is the method of choice to mimic and study the subtleties of neuronal communication either in the intact brain or in slice preparations. However, the currently available caged materials are just at the limit of their usability and have several drawbacks. The local and focal nature of their use may for example be jeopardized by a high spontaneous hydrolysis rate of the commercially available compounds with increased photochemical release rate. Here, using quantum chemical modelling we show the mechanisms of hydrolysis and two-photon activation, and synthesized more effective caged compounds. Furthermore, we have developed a new enzymatic elimination method removing neurotransmitters inadvertently escaping from their compound during experiment. This method, usable both in one and two-photon experiments, allows for the use of materials with an increased rate of photochemical release. The efficiency of the new compound and the enzymatic method and of the new compound are demonstrated in neurophysiological experiments. |
| Databáze: | OpenAIRE |
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