Brain-derived Neurotrophic Factor in the Aqueous Humor of Glaucoma Patients
| Autor: | Igarashi, Tsutomu, Nakamoto, Kenji, Kobayashi, Maika, Suzuki, Hisaharu, Arima, Takeshi, Tobita, Yutaro, Takao, Kazuhiro, Igarashi, Toru, Okuda, Takahisa, Okada, Takashi, Takahashi, Hiroshi, Tsutomu, Igarashi, Kenji, Nakamoto, Maika, Kobayashi, Hisaharu, Suzuki, Takeshi, Arima, Yutaro, Tobita, Kazuhiro, Takao, Toru, Igarashi, Takahisa, Okuda, Takashi, Okada, Hiroshi, Takahashi |
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| Rok vydání: | 2021 |
| Předmět: |
Adult
Male medicine.medical_specialty genetic structures Anterior Chamber medicine.medical_treatment Glaucoma Aqueous humor Enzyme-Linked Immunosorbent Assay Trabeculectomy Pathogenesis Aqueous Humor Elisa kit Neurotrophic factors Ophthalmology medicine Humans Prospective Studies Aged Brain-derived neurotrophic factor Aged 80 and over business.industry Brain-Derived Neurotrophic Factor General Medicine Cataract surgery Middle Aged medicine.disease eye diseases Female sense organs business |
| Zdroj: | Journal of Nippon Medical School = Nippon Ika Daigaku zasshi. 88(2) |
| ISSN: | 1347-3409 |
| Popis: | Background Brain-derived neurotrophic factor (BDNF) may be involved in the pathogenesis of glaucoma. BDNF concentrations reported in previous studies have varied widely, and the concentration of BDNF in aqueous humor is unknown. In this study, BDNF concentrations in the aqueous humor of glaucoma patients and control patients were measured with ELISA kits. Methods This prospective, observational study examined BDNF levels in aqueous humor in 62 eyes of 43 patients who underwent cataract surgery or trabeculectomy (11 glaucoma patients and 32 non-glaucoma cataract patients as controls). BDNF concentrations were examined by 4 different enzyme-linked immunosorbent assay (ELISA) techniques. Results The mean ± SD patient age was 72.0 ± 10.1 (range 35 to 87) years. Two of the techniques detected no BDNF in aqueous humor in any samples (n=3 and n=9, respectively); the average value was less than zero. An ultrasensitive ELISA kit did not yield reliable measurements. Finally, in an even more sensitive ELISA (Simoa-HD1), performed by an outside contractor, 25 (54.3%) eyes were below the detection limit, including 20 (55.6%) control and 5 (50%) glaucoma cases. For eyes with detectable BDNF, the overall BDNF concentration was 0.158 pg/mL (n=21): 0.196 pg/mL (n=16) in controls and 0.034 pg/mL (n=5) in glaucoma cases. Conclusions BDNF level in aqueous humor varies widely. |
| Databáze: | OpenAIRE |
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