Brain-derived Neurotrophic Factor in the Aqueous Humor of Glaucoma Patients

Autor: Igarashi, Tsutomu, Nakamoto, Kenji, Kobayashi, Maika, Suzuki, Hisaharu, Arima, Takeshi, Tobita, Yutaro, Takao, Kazuhiro, Igarashi, Toru, Okuda, Takahisa, Okada, Takashi, Takahashi, Hiroshi, Tsutomu, Igarashi, Kenji, Nakamoto, Maika, Kobayashi, Hisaharu, Suzuki, Takeshi, Arima, Yutaro, Tobita, Kazuhiro, Takao, Toru, Igarashi, Takahisa, Okuda, Takashi, Okada, Hiroshi, Takahashi
Rok vydání: 2021
Předmět:
Zdroj: Journal of Nippon Medical School = Nippon Ika Daigaku zasshi. 88(2)
ISSN: 1347-3409
Popis: Background Brain-derived neurotrophic factor (BDNF) may be involved in the pathogenesis of glaucoma. BDNF concentrations reported in previous studies have varied widely, and the concentration of BDNF in aqueous humor is unknown. In this study, BDNF concentrations in the aqueous humor of glaucoma patients and control patients were measured with ELISA kits. Methods This prospective, observational study examined BDNF levels in aqueous humor in 62 eyes of 43 patients who underwent cataract surgery or trabeculectomy (11 glaucoma patients and 32 non-glaucoma cataract patients as controls). BDNF concentrations were examined by 4 different enzyme-linked immunosorbent assay (ELISA) techniques. Results The mean ± SD patient age was 72.0 ± 10.1 (range 35 to 87) years. Two of the techniques detected no BDNF in aqueous humor in any samples (n=3 and n=9, respectively); the average value was less than zero. An ultrasensitive ELISA kit did not yield reliable measurements. Finally, in an even more sensitive ELISA (Simoa-HD1), performed by an outside contractor, 25 (54.3%) eyes were below the detection limit, including 20 (55.6%) control and 5 (50%) glaucoma cases. For eyes with detectable BDNF, the overall BDNF concentration was 0.158 pg/mL (n=21): 0.196 pg/mL (n=16) in controls and 0.034 pg/mL (n=5) in glaucoma cases. Conclusions BDNF level in aqueous humor varies widely.
Databáze: OpenAIRE