Effect of seminal plasma and sperm of boars valued by freezability on seminal cryopreservation
| Autor: | Henry Mesa, Francisco Javier Henao Uribe, Germán Gómez Londoño, Julián Alonso Valencia Giraldo |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
endocrine system BOAR media_common.quotation_subject Semen Horticulture Biology Cryopreservation Andrology lcsh:Agriculture 03 medical and health sciences 0302 clinical medicine Boar Centrifugation lcsh:Agriculture (General) Acrosome media_common 030219 obstetrics & reproductive medicine urogenital system Reproduction lcsh:S 57 Ciencias de la vida Biología / Life sciences biology Forestry Seminal quality Anatomy 58 Plantas / Plants Sperm lcsh:S1-972 030104 developmental biology DNA fragmentation Animal Science and Zoology Seminal preservation Agronomy and Crop Science Food Science |
| Zdroj: | Revista Facultad Nacional de Agronomía Medellín, Vol 69, Iss 2, Pp 7903-7910 (2016) Redalyc Repositorio UN Universidad Nacional de Colombia instacron:Universidad Nacional de Colombia |
| ISSN: | 2248-7026 0304-2847 |
| Popis: | The aim of this study was to determine the effect of sperm and seminal plasma (SP) on the freezability of porcine semen. Semen of eight commercial males from two farms in the central-western region of Colombia (four boars in each farm) was frozen and tested to select two males with high freezability (MHF) and two with low freezability (MLF), according to the percentage of functionally competent sperm (FCS). Immediately after the collection was completed, the SP and sperm from the males selected were separated by centrifugation to combine the two types of plasma with the two types of sperm, incubate them for three hours and then freeze them. The variables evaluated were: sperm morphology, structural and functional integrity of plasmatic membrane, progressive and total motility, DNA fragmentation, acrosome integrity, capacitated sperm and FCS. The combination of sperm and plasma of MHF recorded the highest value (P |
| Databáze: | OpenAIRE |
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